Overview

  • Product nameAnti-PIGT antibody
    See all PIGT primary antibodies
  • Description
    Rabbit polyclonal to PIGT
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 432-481 (IQLPANSVTK VSIQFERALL KWTEYTPDPN HGFYVSPSVL SALVPSMVAA) of Human PIGT (NP_057021).

  • Positive control
    • Transfected 293T cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab80888 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 66 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

Target

  • RelevancePIGT is an essential component of the multisubunit enzyme, GPI transamidase and is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells which serves to anchor proteins to the cell surface. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins.
  • Cellular localizationEndoplasmic reticulum membrane; Single-pass type I membrane protein.
  • Database links
  • Alternative names
    • CGI 06 antibody
    • FLJ41596 antibody
    • GPI transamidase component PIG T antibody
    • GPI transamidase subunit antibody
    • MGC8909 antibody
    • NDAP antibody
    • Neurotrophin regulated neuronal development associated protein antibody
    • Phosphatidyl inositol glycan class T antibody
    • Phosphatidylinositol glycan anchor biosynthesis class T antibody
    • Phosphatidylinositol glycan class T antibody
    see all

Anti-PIGT antibody images

  • Anti-PIGT antibody (ab80888) at 1 µg/ml (in 5% skim milk / PBS buffer) + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 66 kDa
    Observed band size : 64 kDa (why is the actual band size different from the predicted?)

References for Anti-PIGT antibody (ab80888)

ab80888 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"