Overview

  • Product name
  • Description
    Rabbit polyclonal to PIGV
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 51-100 (FVDQLVEGLL GGLSHWDAEH FLFIAEHGYL YEHNFAFFPG FPLALLVGTE) of Human PIGV (NP_060307)

  • Positive control
    • Jurkat cell lysate, Human kidney tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab105697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 5 µg/ml. Predicted molecular weight: 56 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Alpha-1,6-mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the second mannose to the glycosylphosphatidylinositol during GPI precursor assembly.
  • Pathway
    Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
  • Involvement in disease
    Defects in PIGV are the cause of hyperphosphatasia with mental retardation (HPMR) [MIM:239300]. It is a syndrome characterized by elevated serum alkaline phosphatase, severe mental retardation, seizures, hypotonia, facial dysmorphism, and hypoplastic terminal phalanges.
  • Sequence similarities
    Belongs to the PIGV family.
  • Post-translational
    modifications
    Not N-glycosylated.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • GPI mannosyltransferase 2 antibody
    • GPI mannosyltransferase II antibody
    • GPI MT II antibody
    • GPI-MT-II antibody
    • Phosphatidylinositol glycan biosynthesis class V protein antibody
    • Phosphatidylinositol-glycan biosynthesis class V protein antibody
    • PIG-V antibody
    • Pigv antibody
    • PIGV_HUMAN antibody
    see all

Images

  • Paraffin embedded Human kidney tissue labelled with ab105697 at 8 µg/ml. Positively labelled epithelial cells of the renal tubule are indicated with arrows.
  • All lanes : Anti-PIGV antibody (ab105697) at 5 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : Jurkat cell lysate at 10 µg


    Predicted band size : 56 kDa

References

ab105697 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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