Overview

  • Product nameAnti-PIGY antibody
    See all PIGY primary antibodies
  • Description
    Rabbit polyclonal to PIGY
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    amino acids 23-114 (VARRCLHASG SRPLADRGKK TEEPPRDFDP ALLEFLVCPL SKKPLRYEAS TNELINEELG IAYPIIDGIP NMIPQAARMT RQSKKQEEVE QR) of Human PIGY.

  • Positive control
    • Human adrenal gland tissue; RT-4, U-251 MG, Human liver and tonsil lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • RelevanceThis gene encodes two proteins, one of which is part of the GPI-N-acetylglucosaminyltransferase (GIP-GnT) complex which initiates the biosynthesis of glycosylphosphatidylinositol (GPI). GPI is synthesized in the endoplasmic reticulum and serves as an anchor for many surface proteins. Proteins containing GPI anchors can have an important role in cell-cell interactions. Two open reading frames have been found in the single transcript that has been identified for this gene. The downstream open reading frame encodes the GPI-GnT complex protein while the upstream open reading frame encodes a protein with unknown function.
  • Cellular localizationEndoplasmic reticulum membrane. Plasma membrane.
  • Database links
  • Alternative names
    • Phosphatidylinositol glycan anchor biosynthesis, class Y antibody
    • Phosphatidylinositol N-acetylglucosaminyltransferase subunit Y antibody
    • Phosphatidylinositol-glycan biosynthesis class Y protein antibody
    • PIG Y antibody
    • PIG-Y antibody
    see all

Anti-PIGY antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and mitochondria. Recommended concentration of ab122338 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122338, at a 1/75 dilution, staining PIGY in paraffin-embedded Human adrenal gland tissue by immunohistochemistry.
  • All lanes : Anti-PIGY antibody (ab122338) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique

References for Anti-PIGY antibody (ab122338)

ab122338 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"