Overview

  • Product name
  • Description
    Rabbit polyclonal to PIGY
  • Specificity
    Only detects isoform 1 of PIGY.
  • Tested applications
    Suitable for: ICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    16 amino acid synthetic peptide from near the center of Human PIGY

  • Positive control
    • WB: Human liver and spleen tissue lysate. IF: Human spleen tissue. IHC-P: Human spleen tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab79453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 8 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • Relevance
    This gene encodes two proteins, one of which is part of the GPI-N-acetylglucosaminyltransferase (GIP-GnT) complex which initiates the biosynthesis of glycosylphosphatidylinositol (GPI). GPI is synthesized in the endoplasmic reticulum and serves as an anchor for many surface proteins. Proteins containing GPI anchors can have an important role in cell-cell interactions. Two open reading frames have been found in the single transcript that has been identified for this gene. The downstream open reading frame encodes the GPI-GnT complex protein while the upstream open reading frame encodes a protein with unknown function.
  • Cellular localization
    Endoplasmic reticulum membrane. Plasma membrane.
  • Database links
  • Alternative names
    • Phosphatidylinositol glycan anchor biosynthesis, class Y antibody
    • Phosphatidylinositol N-acetylglucosaminyltransferase subunit Y antibody
    • Phosphatidylinositol-glycan biosynthesis class Y protein antibody
    • PIG Y antibody
    • PIG-Y antibody
    see all

Anti-PIGY antibody images

  • Immunofluorescence of PIGY in Human spleen tissue with ab79453 at 20 ug/mL.

  • Immunohistochemistry of Human spleen tissue using ab79453 at 2.5 μg/mL.

  • Lane 1 : Anti-PIGY antibody (ab79453) at 1 µg/ml
    Lane 2 : Anti-PIGY antibody (ab79453) at 2 µg/ml

    Lane 1 : human spleen tissue lysate
    Lane 2 : human spleen tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 8 kDa
    Despite its predicted molecular weight, PIGY often migrates at 28-30 kDa in SDS-PAGE.
  • Lane 1 : Anti-PIGY antibody (ab79453) at 1 µg/ml
    Lane 2 : Anti-PIGY antibody (ab79453) at 2 µg/ml

    Lane 1 : Human liver tissue lysate
    Lane 2 : Human liver tissue lysate


    Predicted band size : 8 kDa

References for Anti-PIGY antibody (ab79453)

ab79453 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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