Overview

  • Product nameAnti-PIGY antibody
    See all PIGY primary antibodies
  • Description
    Rabbit polyclonal to PIGY
  • SpecificityThis antibody only detects isoform 1 of PIGY.
  • Tested applicationsSuitable for: ICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A 16 amino acid peptide near the carboxy terminus of human PIGY (NP_116295)

  • Positive control
    • A-20 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAffinity chromatography purified via peptide column
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab79510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 8 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • RelevanceThis gene encodes two proteins, one of which is part of the GPI-N-acetylglucosaminyltransferase (GIP-GnT) complex which initiates the biosynthesis of glycosylphosphatidylinositol (GPI). GPI is synthesized in the endoplasmic reticulum and serves as an anchor for many surface proteins. Proteins containing GPI anchors can have an important role in cell-cell interactions. Two open reading frames have been found in the single transcript that has been identified for this gene. The downstream open reading frame encodes the GPI-GnT complex protein while the upstream open reading frame encodes a protein with unknown function.
  • Cellular localizationEndoplasmic reticulum membrane. Plasma membrane.
  • Database links
  • Alternative names
    • Phosphatidylinositol glycan anchor biosynthesis, class Y antibody
    • Phosphatidylinositol N-acetylglucosaminyltransferase subunit Y antibody
    • Phosphatidylinositol-glycan biosynthesis class Y protein antibody
    • PIG Y antibody
    • PIG-Y antibody
    see all

Anti-PIGY antibody images

  • Immunofluorescence of PIGY in Human spleen tissue with ab79510 at 20 ug/mL.

  • Immunohistochemistry of Human spleen tissue using ab79510 at 2.5 μg/mL.

  • Anti-PIGY antibody (ab79510) at 2 µg/ml + A-20 cell lysate at 15 µg

    Predicted band size : 8 kDa
    Observed band size : 28-30 kDa (why is the actual band size different from the predicted?)
    Despite its predicted molecular weight, PIGY often migrates at 28-30 kDa in SDS-PAGE.

References for Anti-PIGY antibody (ab79510)

ab79510 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"