Overview

  • Product nameAnti-PIH1D1 antibody
    See all PIH1D1 primary antibodies
  • Description
    Mouse monoclonal to PIH1D1
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length human PIH1D1 (AAH01108) with a proprietary tag.

  • Positive control
    • WB: the immunogen; IMR32 cell lysate ICC/IF: HeLa cell

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab89143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 32 kDa.
ELISA Use at an assay dependent dilution. The detection limit is approximately 10ng/ml when sued as capture antibody.
ICC/IF Use a concentration of 10 µg/ml.

Target

Anti-PIH1D1 antibody images

  • Anti-PIH1D1 antibody (ab89143) at 5 µg/ml + tagged, recombinant human PIH1D1 protein (the immunogen) at 0.2 µg

    Predicted band size : 32 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
  • Anti-PIH1D1 antibody (ab89143) at 5 µg/ml + IMR32 cell lysate at 50 µg

    Predicted band size : 32 kDa
    Observed band size : 32 kDa
  • ab89143, at 10 µg/ml, staining PIH1D1 in HeLa cell by Immunofluorescence.

References for Anti-PIH1D1 antibody (ab89143)

ab89143 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"