Overview

  • Product nameAnti-PIK3IP1 antibody
    See all PIK3IP1 primary antibodies
  • Description
    Rabbit polyclonal to PIK3IP1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from within residues: ALPAFTTEIQ EASEGPGADE VQVFAPANAL PARSEAAAVQ PVIGISQRVR , corresponding to internal sequence amino acids 108-157 of Human PIK3IP1 (NP_443112).

  • Positive control
    • HCT15 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab87094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa.Can be blocked with PIK3IP1 peptide (ab135922). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Sequence similaritiesContains 1 kringle domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • HGFL antibody
    • hHGFL(S) antibody
    • Kringle domain containing protein HGFL antibody
    • Kringle domain-containing protein HGFL antibody
    • MGC17330 antibody
    • P3IP1_HUMAN antibody
    • Phosphoinositide 3 kinase interacting protein 1 antibody
    • Phosphoinositide-3-kinase-interacting protein 1 antibody
    • PIK3IP1 antibody
    see all

Anti-PIK3IP1 antibody images

  • Anti-PIK3IP1 antibody (ab87094) at 1 µg/ml + HCT15 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 28 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-PIK3IP1 antibody (ab87094) at 1/1000 dilution (in TBST + 5% Milk for 16 hours at 4°C)

    Lane 1 : Whole cell lysate of Human U87MG treated with DMSO
    Lane 2 : Whole cell lysate of Human U87MG treated with 1µM LY294002 for 6 hours
    Lane 3 : Whole cell lysate of Human U87MG treated with 10µM LY294002 for 6 hours

    Lysates/proteins at 50 µg per lane.

    Secondary
    An HRP-conjugated Goat anti-rabbit polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 28 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-PIK3IP1 antibody (ab87094)

This product has been referenced in:
  • Yap TA  et al. AT13148 is a novel, oral multi-AGC kinase inhibitor with potent pharmacodynamic and antitumor activity. Clin Cancer Res 18:3912-23 (2012). WB ; Human . Read more (PubMed: 22781553) »
  • Alexander MS  et al. Regulation of DMD pathology by an ankyrin-encoded miRNA. Skelet Muscle 1:27 (2011). WB . Read more (PubMed: 21824387) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us. The blocking peptide for ab87094 has been added to our catalog as ab135922. Please let me know if you need any additional information or assistance.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (U87MG tumour cells)
Loading amount 50 µg
Specification U87MG tumour cells
Treatment 1 & 10UM LY294002 for 6h
Gel Running Conditions Reduced Denaturing (10% Tris Glycine)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Sep 16 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"