The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
FunctionRegulatory subunit of the PI3K gamma complex.
Tissue specificityUbiquitously expressed with high expression in fetal brain compared to adult brain. Abundant expression is observed in cerebellum, cerebral cortex, cerebral meninges, and vermis cerebelli.
Involvement in diseaseNote=Defects in PIK3R5 may be a cause of autosomal recessive ataxia with oculomotor apraxia (AOA). A PIK3R5 mutation segregates with the disease phenotype in a family affected by gait ataxia, cerebellar dysarthric speech, and impaired ocular movement. Additional features include reduced or absent tendon reflexes, severe cerebellar atrophy, marked vermal atrophy, moderately severe axonal sensory polyneuropathy with absent sensory nerve action potential in the lower extremities, and persistently elevated alpha-fetoprotein levels.
DomainThe heterodimerization region allows the binding to the catalytic subunit.
Anti-PIK3R5 antibody (ab62792) at 1/100 dilution + K562 cell line lysates at 35 µg
Predicted band size : 97 kDa Observed band size : 97 kDa Additional bands at : 55 kDa,75 kDa. We are unsure as to the identity of these extra bands.
References for Anti-PIK3R5 antibody (ab62792)
This product has been referenced in:
Wilson LS et al. A phosphodiesterase 3B-based signaling complex integrates exchange protein activated by cAMP 1 and phosphatidylinositol 3-kinase signals in human arterial endothelial cells. J Biol Chem286:16285-96 (2011).
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