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Rabbit polyclonal to Pikachurin
Predicted to work with:
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Rat Pikachurin.
This antibody gave a positive signal in Mouse eye (Retina) FFPE section.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Secreted › extracellular space › extracellular matrix By similarity. Cell junction › synapse
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pikachurin antibody (ab91314)
IHC image of Pikachurin staining in Mouse eye (Retina) FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91314, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
has not yet been referenced specifically in any publications.
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