Overview

  • Product name
  • Description
    Rabbit polyclonal to PIM1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MLLSKINSLA HLRAAPCNDL HATKLAPGKE KEPLESQYQV GPLLGSGGFG) of Human PIM1 (NP_002639).

  • Positive control
    • Fetal heart lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab98004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Isoform 2 promotes the G1/S transition of the cell cycle via up-regulation of CDK2 activity and phosphorylation of CDKN1B, resulting in enhanced nuclear export and proteasome-dependent degradation of CDKN1B. Isoform 2 also represses CDKN1B transcription by phosphorylating and inactivating the transcription factor FOXO3. Plays a role in signal transduction in blood cells. Contributes to both cell proliferation and survival and thus provides a selective advantage in tumorigenesis.
  • Tissue specificity
    Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated on both serine/threonine and tyrosine residues.
  • Cellular localization
    Cytoplasm. Nucleus and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Oncogene PIM 1 antibody
    • Oncogene PIM1 antibody
    • PIM 1 antibody
    • pim 1 kinase 44 kDa isoform antibody
    • Pim 1 kinase antibody
    • pim 1 oncogene (proviral integration site 1) antibody
    • Pim 1 oncogene antibody
    • PIM antibody
    • PIM1 antibody
    • pim1 kinase 44 kDa isoform antibody
    • PIM1_HUMAN antibody
    • Pim2 antibody
    • PIM3 antibody
    • Proto oncogene serine/threonine protein kinase Pim 1 antibody
    • Proto-oncogene serine/threonine-protein kinase Pim-1 antibody
    • Proviral integration site 1 antibody
    • Proviral integration site 2 antibody
    see all

Images

  • Anti-PIM1 antibody (ab98004) at 1 µg/ml (5% skim milk/ PBS buffer) + fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 36 kDa

References

ab98004 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab98004.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up