Synthetic peptide derived from the internal region of the human PIM1 protein, which is 94% and 88% homologous with rat and mouse, respectively.
Our Abpromise guarantee covers the use of ab54503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 35 kDa.|
|ELISA||Use a concentration of 0.1 - 1 µg/ml.|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PIM1 knockout HAP1 cell lysate (20 µg)
Lane 3: K562 cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab54503 observed at 35 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab54503 was shown to recognize PIM1 when PIM1 knockout samples were used, along with additional cross-reactive bands. Wild-type and PIM1 knockout samples were subjected to SDS-PAGE. ab54503 and ab181602 (loading control to GAPDH) were diluted 3 μg/mL and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
ab54503 has not yet been referenced specifically in any publications.