Anti-PIM1 (phospho Y309) antibody (ab74047)


  • Product nameAnti-PIM1 (phospho Y309) antibody
    See all PIM1 primary antibodies
  • Description
    Rabbit polyclonal to PIM1 (phospho Y309)
  • Specificityab74047 detects endogenous levels of human PIM1 only when phosphorylated at tyrosine 309. ab74047 detects endogenous levels of mouse PIM1 only when phosphorylated at tyrosine 302 and rat PIM1 only when phosphorylated at tyrosine 218.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human PIM1 around the phosphorylation site of tyrosine 309 (H-R-YP-H-G).

  • Positive control
    • HUVEC cell extracts treated with PMA (125ng/ml, 30mins).



Our Abpromise guarantee covers the use of ab74047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
ELISA 1/40000.


  • FunctionMay affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Isoform 2 promotes the G1/S transition of the cell cycle via up-regulation of CDK2 activity and phosphorylation of CDKN1B, resulting in enhanced nuclear export and proteasome-dependent degradation of CDKN1B. Isoform 2 also represses CDKN1B transcription by phosphorylating and inactivating the transcription factor FOXO3. Plays a role in signal transduction in blood cells. Contributes to both cell proliferation and survival and thus provides a selective advantage in tumorigenesis.
  • Tissue specificityExpressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    Autophosphorylated on both serine/threonine and tyrosine residues.
  • Cellular localizationCytoplasm. Nucleus and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Oncogene PIM 1 antibody
    • Oncogene PIM1 antibody
    • PIM 1 antibody
    • pim 1 kinase 44 kDa isoform antibody
    • Pim 1 kinase antibody
    • pim 1 oncogene (proviral integration site 1) antibody
    • Pim 1 oncogene antibody
    • PIM antibody
    • PIM1 antibody
    • pim1 kinase 44 kDa isoform antibody
    • PIM1_HUMAN antibody
    • Pim2 antibody
    • PIM3 antibody
    • Proto oncogene serine/threonine protein kinase Pim 1 antibody
    • Proto-oncogene serine/threonine-protein kinase Pim-1 antibody
    • Proviral integration site 1 antibody
    • Proviral integration site 2 antibody
    see all

Anti-PIM1 (phospho Y309) antibody images

  • All lanes : Anti-PIM1 (phospho Y309) antibody (ab74047) at 1/500 dilution

    Lane 1 : HUVEC cell extracts treated with PMA (125ng/ml, 30mins)
    Lane 2 : HUVEC cell extracts treated with PMA (125ng/ml, 30mins) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 35 kDa
    Observed band size : 35 kDa

References for Anti-PIM1 (phospho Y309) antibody (ab74047)

ab74047 has not yet been referenced specifically in any publications.

Product Wall

The Pim1 antibodies we have in our catalog are : ab54457 : the epitope recognized by this antibody maps to a region between aa 250-313 and is specific to Pim-1. No cross-reactivity with Pim-2 or -3 is expected. ab74047 and ab74118 can't react with ...

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