Overview

  • Product name
  • Description
    Goat polyclonal to PIM2
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human PIM2 aa 19-31 (internal sequence) (Cysteine residue). NP_006866.2.
    Sequence:

    PPGGKDREAFEAE


    Database link: Q9P1W9

  • Positive control
    • HEK293 transiently expressing Human PIM2 (myc and DYKDDDDK tagged).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab176350 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab176350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa).

Target

  • Function
    Promotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappaB kinase/NF-kappaB cascade; this process requires phosphorylation of MAP3K8/COT. Prevents apoptosis induced by growth factor withdrawal via inhibition of caspase-3 activation, and via phosphorylation of pro-apoptotic proteins. Inhibits BAD-induced cell death via phosphorylation of BAD. PIM2-mediated cell survival is glucose-dependent but independent of several AKT regulators such as PI3K, HSP-90 and TOR, indicating that PIM2 and PI3K/AKT/TOR function via distinct pathways. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate.
  • Tissue specificity
    Highly expressed in hematopoietic tissues, in leukemic and lymphoma cell lines, testis, small intestine, colon and colorectal adenocarcinoma cells. Weakly expressed in normal liver, but highly expressed in hepatocellular carcinoma tissues.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Information by UniProt
  • Database links
  • Alternative names
    • OTTHUMP00000025825 antibody
    • Pim 2 oncogene antibody
    • Pim 2h antibody
    • Pim-2h antibody
    • PIM2 antibody
    • PIM2 Oncogene antibody
    • PIM2_HUMAN antibody
    • Pim2h antibody
    • Proto Oncogene PIM2 antibody
    • Proto-oncogene Pim-2 (serine threonine kinase) antibody
    • Serine/threonine kinase PIM2 antibody
    • Serine/threonine-protein kinase Pim-2 antibody
    see all

Images

  • Lanes 1-2 : Anti-PIM2 antibody (ab176350) at 1 mg/ml
    Lane 3 : anti-MYC Tag at 1/1000 dilution

    Lanes 1 & 3 : HEK293 lysate overexpressing Human PIM2 with C-terminal MYC tag
    Lane 2 : Mock-transfected HEK293 lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 34 kDa

References

ab176350 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab176350.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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