Overview

  • Product nameAnti-PIM2 antibody
    See all PIM2 primary antibodies
  • Description
    Rabbit polyclonal to PIM2
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 53-248 of Human PIM2 (AAH18111)

  • Positive control
    • 293T, A431, HeLa, HepG2 and Molt-4 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab97475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 34 kDa.
ICC/IF 1/100 - 1/200.

Target

  • FunctionPromotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappaB kinase/NF-kappaB cascade; this process requires phosphorylation of MAP3K8/COT. Prevents apoptosis induced by growth factor withdrawal via inhibition of caspase-3 activation, and via phosphorylation of pro-apoptotic proteins. Inhibits BAD-induced cell death via phosphorylation of BAD. PIM2-mediated cell survival is glucose-dependent but independent of several AKT regulators such as PI3K, HSP-90 and TOR, indicating that PIM2 and PI3K/AKT/TOR function via distinct pathways. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate.
  • Tissue specificityHighly expressed in hematopoietic tissues, in leukemic and lymphoma cell lines, testis, small intestine, colon and colorectal adenocarcinoma cells. Weakly expressed in normal liver, but highly expressed in hepatocellular carcinoma tissues.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Information by UniProt
  • Database links
  • Alternative names
    • OTTHUMP00000025825 antibody
    • Pim 2 oncogene antibody
    • Pim 2h antibody
    • Pim-2h antibody
    • PIM2 antibody
    • PIM2 Oncogene antibody
    • PIM2_HUMAN antibody
    • Pim2h antibody
    • Proto Oncogene PIM2 antibody
    • Proto-oncogene Pim-2 (serine threonine kinase) antibody
    • Serine/threonine kinase PIM2 antibody
    • Serine/threonine-protein kinase Pim-2 antibody
    see all

Anti-PIM2 antibody images

  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97475 at 1/200 dilution (1) and with Hoescht nuclear stain (2).
  • Anti-PIM2 antibody (ab97475) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size : 34 kDa

References for Anti-PIM2 antibody (ab97475)

This product has been referenced in:
  • Huot G  et al. CHES1/FOXN3 regulates cell proliferation by repressing PIM2 and protein biosynthesis. Mol Biol Cell 25:554-65 (2014). WB ; Human . Read more (PubMed: 24403608) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"