Overview

  • Product nameAnti-PIM3 antibody
    See all PIM3 primary antibodies
  • Description
    Rabbit polyclonal to PIM3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Macaque Monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human PIM3.

  • Positive control
    • This antibody gave a positive signal in Human Heart tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab108920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 35 kDa).

Target

  • FunctionAble to phosphorylate CDKN1B in vitro. Involved in cell cycle progression and suppression of apoptosis. Implicated in proliferation of human hepatoma cell lines.
  • Tissue specificityWidely expressed. No expression in colon, thymus, and small intestine. Expressed in human hepatoma cell lines but not in normal liver tissues. Expressed in colon cancer samples, particularly at the less advanced stages of cancer development.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Kid 1 antibody
    • Kid1 antibody
    • Kinase induced by depolarization antibody
    • ONCOGENE PIM3 antibody
    • PIM 3 antibody
    • Pim 3 oncogene antibody
    • Pim-3 proto-oncogene, serine/threonine kinase antibody
    • PIM3 antibody
    • Pim3 oncogene antibody
    • PIM3_HUMAN antibody
    • Protein kinase Kid 1 antibody
    • Protein kinase Kid1 antibody
    • Serine/threonine kinase Pim 3 antibody
    • Serine/threonine kinase Pim3 antibody
    • Serine/threonine protein kinase Pim 3 antibody
    • Serine/threonine protein kinase Pim3 antibody
    • Serine/threonine-protein kinase Pim-3 antibody
    see all

Anti-PIM3 antibody images

  • All lanes : Anti-PIM3 antibody (ab108920) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human heart tissue lysate - total protein (ab29431) with Immunizing peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 73 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

References for Anti-PIM3 antibody (ab108920)

ab108920 has not yet been referenced specifically in any publications.

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