The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa).
Use at 2-5 µg/mg of lysate.
FunctionCatalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m(7)G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs). Plays a role in transcriptional regulation.
Tissue specificityUbiquitously expressed. High expression in heart, skeletal muscle, kidney, liver and placenta.
Sequence similaritiesBelongs to the methyltransferase superfamily. Trimethylguanosine synthase family.
Cellular localizationCytoplasm. Nucleus > Cajal body. A 90 kDa isoform is found in the nucleus while a 55 kDa isoform is found in the cytoplasm and colocalizes with the tubulin network.
Detection of Human PIMT by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70559 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
References for Anti-PIMT antibody (ab70559)
has not yet been referenced specifically in any publications.
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