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Mouse monoclonal [3G8] to Pin1
Recombinant fragment, corresponding to amino acids 1/163 of Human Pin1
293T, HeLa and Jurkat cells.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4
Concentration information loading...
Protein G purified
Light chain type
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 18 kDa.
Use at an assay dependent dilution.
Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzing pSer/Thr-Pro cis/trans isomerizations.
Contains 1 PpiC domain.
Contains 1 WW domain.
The WW domain is required for the interaction with STIL and KIF20B.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
DODO, Drosophila, homolog of antibody
Western blot - Pin1 antibody [3G8] (ab38933)
All lanes :
Anti-Pin1 antibody [3G8] (ab38933) at 1/500 dilution
Lane 1 :
293T cell lysate
Lane 2 :
HeLa cell lysate
Lane 3 :
Jurkat cell lysate
Lysates/proteins at 50 µg per lane.
Secondary All lanes :
goat anti-mouse secondary antibody conjugated to HRP
Developed using the ECL technique.
Predicted band size:
Observed band size:
16 kDa (
why is the actual band size different from the predicted?
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"