The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 10 µg/mg of lysate.
Is unsuitable for WB.
Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzing pSer/Thr-Pro cis/trans isomerizations.
Contains 1 PpiC domain. Contains 1 WW domain.
The WW domain is required for the interaction with STIL and KIF20B.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Detection of Pin1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab113946 at 10 µg/mg lysate for IP. An anti-Pin1 antibody which recognizes a downstream epitope was used at 1 µg/ml for subsequent western blot detection. Detection: Chemiluminescence with exposure time of 3 minutes.