The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 10 µg/mg of lysate.
Application notesIs unsuitable for WB.
FunctionEssential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzing pSer/Thr-Pro cis/trans isomerizations.
Peptidylprolyl cis/trans isomerase NIMA interacting 1 antibody
Pin 1 antibody
PPIase Pin1 antibody
Prolyl isomerase antibody
Protein (peptidylprolyl cis/trans isomerase) NIMA interacting 1 antibody
Protein NIMA interacting 1 antibody
Rotamase Pin1 antibody
UBL 5 antibody
Anti-Pin1 antibody images
Immunoprecipitation - Pin1 antibody (ab113946)
Detection of Pin1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab113946 at 10 µg/mg lysate for IP. An anti-Pin1 antibody which recognizes a downstream epitope was used at 1 µg/ml for subsequent western blot detection. Detection: Chemiluminescence with exposure time of 3 minutes.
References for Anti-Pin1 antibody (ab113946)
has not yet been referenced specifically in any publications.
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