Overview

  • Product name
  • Description
    Rabbit polyclonal to Pin1
  • Specificity
    ab12107 detects human Pin 1 at ~20kDa. ab12107 also detects a band at ~23kDa, which is not attenuated by blocking peptide ab12397; the identity of this band is unknown.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from residues 50 - 150 of Human Pin1.

    .

  • Positive control
    • This antibody gave a positive result with Pin1 recombinant protein

Properties

Applications

Our Abpromise guarantee covers the use of ab12107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 18.2 kDa).

Target

  • Function
    Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzing pSer/Thr-Pro cis/trans isomerizations.
  • Sequence similarities
    Contains 1 PpiC domain.
    Contains 1 WW domain.
  • Domain
    The WW domain is required for the interaction with STIL and KIF20B.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DOD antibody
    • DODO, Drosophila, homolog of antibody
    • FLJ40239 antibody
    • FLJ77628 antibody
    • MGC10717 antibody
    • NIMA interacting 1 antibody
    • Peptidyl prolyl cis trans isomerase NIMA interacting 1 antibody
    • Peptidyl prolyl cis/trans isomerase NIMA interacting antibody
    • Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 antibody
    • Peptidyl-prolyl cis-trans isomerase pin1 antibody
    • Peptidylprolyl cis/trans isomerase NIMA interacting 1 antibody
    • Pin 1 antibody
    • Pin1 antibody
    • PIN1_HUMAN antibody
    • PPIase Pin1 antibody
    • Prolyl isomerase antibody
    • Protein (peptidylprolyl cis/trans isomerase) NIMA interacting 1 antibody
    • Protein NIMA interacting 1 antibody
    • Rotamase Pin1 antibody
    • UBL 5 antibody
    • UBL5 antibody
    see all

Anti-Pin1 antibody images

  • Lane 1 : Marker
    Lanes 2 - 3 : Anti-Pin1 antibody (ab12107) at 1 µg/ml

    Lane 1 : As above
    Lane 2 : pin1 Recombinant 0.1ug
    Lane 3 : pin1 Recombinant 0.5ug

    Secondary
    Lanes 2 - 3 : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/10000 dilution


    Performed under reducing conditions.

    Predicted band size : 18.2 kDa
    Observed band size : ~34 kDa (why is the actual band size different from the predicted?)
    The observed band size is consistent with the expected size of the Pin1 partial recombinant protein used (amino acids 64 - 163 of the human Pin1 sequence, with GST).

References for Anti-Pin1 antibody (ab12107)

This product has been referenced in:
  • Bhaskaran N  et al. Fbw7a and Fbw7? collaborate to shuttle cyclin E1 into the nucleolus for multiubiquitylation. Mol Cell Biol 33:85-97 (2013). Human . Read more (PubMed: 23109421) »
  • Lv L  et al. Inhibition of peptidyl-prolyl cis/trans isomerase Pin1 induces cell cycle arrest and apoptosis in vascular smooth muscle cells. Apoptosis 15:41-54 (2010). WB ; Human . Read more (PubMed: 19904610) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

Submitted Mar 17 2009

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

Submitted Jan 28 2009

Thank you for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you d...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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