Overview

  • Product name
  • Description
    Rabbit polyclonal to Pin1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-163 of Human Pin1 (UniProt ID: Q13526).

  • Positive control
    • A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates; A549 cells
  • General notes
    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab154577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 18 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzing pSer/Thr-Pro cis/trans isomerizations.
  • Sequence similarities
    Contains 1 PpiC domain.
    Contains 1 WW domain.
  • Domain
    The WW domain is required for the interaction with STIL and KIF20B.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DOD antibody
    • DODO, Drosophila, homolog of antibody
    • FLJ40239 antibody
    • FLJ77628 antibody
    • MGC10717 antibody
    • NIMA interacting 1 antibody
    • Peptidyl prolyl cis trans isomerase NIMA interacting 1 antibody
    • Peptidyl prolyl cis/trans isomerase NIMA interacting antibody
    • Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 antibody
    • Peptidyl-prolyl cis-trans isomerase pin1 antibody
    • Peptidylprolyl cis/trans isomerase NIMA interacting 1 antibody
    • Pin 1 antibody
    • Pin1 antibody
    • PIN1_HUMAN antibody
    • PPIase Pin1 antibody
    • Prolyl isomerase antibody
    • Protein (peptidylprolyl cis/trans isomerase) NIMA interacting 1 antibody
    • Protein NIMA interacting 1 antibody
    • Rotamase Pin1 antibody
    • UBL 5 antibody
    • UBL5 antibody
    see all

Images

  • All lanes : Anti-Pin1 antibody (ab154577) at 1/1000 dilution

    Lane 1 : HepG2 whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 18 kDa



    12% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed A549 cell labeling Pin1 with ab154577 at 1/200 dilution. The lower panel was merged with DNA probe.

References

ab154577 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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