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Rabbit polyclonal to Pin1
Predicted to work with:
Cow, Xenopus tropicalis
Recombinant fragment, corresponding to a region within amino acids 1-163 of Human Pin1 (UniProt ID: Q13526).
A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates; A549 cells
Keep as concentrated solution.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 18 kDa.
1/100 - 1/1000.
Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzing pSer/Thr-Pro cis/trans isomerizations.
Contains 1 PpiC domain.
Contains 1 WW domain.
The WW domain is required for the interaction with STIL and KIF20B.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
DODO, Drosophila, homolog of antibody
Western blot - Anti-Pin1 antibody (ab154577)
All lanes : Anti-Pin1 antibody (ab154577) at 1/1000 dilution Lane 1 : HepG2 whole cell lysate Lane 2 : Raji whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size: 18 kDa 12% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-Pin1 antibody (ab154577)
Immunofluorescence analysis of paraformaldehyde-fixed A549 cell labeling Pin1 with ab154577 at 1/200 dilution. The lower panel was merged with DNA probe.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"