Recombinant
RabMAb

Anti-Pin1 (phospho S16) antibody [EP1480Y] (ab62378)

Overview

  • Product name
    Anti-Pin1 (phospho S16) antibody [EP1480Y]
    See all Pin1 primary antibodies
  • Description
    Rabbit monoclonal [EP1480Y] to Pin1 (phospho S16)
  • Specificity
    This antibody detects Pin1 phosphorylated at Serine 16.
  • Tested applications
    Suitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Phospho specific peptide corresponding to residues surround Serine 16 of human Pin1.

  • Positive control
    • HeLa cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab62378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzing pSer/Thr-Pro cis/trans isomerizations.
    • Sequence similarities
      Contains 1 PpiC domain.
      Contains 1 WW domain.
    • Domain
      The WW domain is required for the interaction with STIL and KIF20B.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • DOD antibody
      • DODO, Drosophila, homolog of antibody
      • FLJ40239 antibody
      • FLJ77628 antibody
      • MGC10717 antibody
      • NIMA interacting 1 antibody
      • Peptidyl prolyl cis trans isomerase NIMA interacting 1 antibody
      • Peptidyl prolyl cis/trans isomerase NIMA interacting antibody
      • Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 antibody
      • Peptidyl-prolyl cis-trans isomerase pin1 antibody
      • Peptidylprolyl cis/trans isomerase NIMA interacting 1 antibody
      • Pin 1 antibody
      • Pin1 antibody
      • PIN1_HUMAN antibody
      • PPIase Pin1 antibody
      • Prolyl isomerase antibody
      • Protein (peptidylprolyl cis/trans isomerase) NIMA interacting 1 antibody
      • Protein NIMA interacting 1 antibody
      • Rotamase Pin1 antibody
      • UBL 5 antibody
      • UBL5 antibody
      see all

    Images

    • All lanes : Anti-Pin1 (phospho S16) antibody [EP1480Y] (ab62378) at 1/500 dilution

      Lane 1 : HeLa cell lysate (untreated)
      Lane 2 : HeLa cell lysate (treated with FBS + Calyculin A)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP conjugated at 1/2000 dilution

      Predicted band size : 18 kDa
      Observed band size : 18 kDa

    References

    ab62378 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
    Permeabilization
    Yes - Wash Buffer from Dako with Tween
    Specification
    Liver
    Fixative
    Paraformaldehyde
    Username

    Mr. Rudolf Jung

    Verified customer

    Submitted Oct 20 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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