Anti-Pin1 (phospho S16) antibody [EP1480Y] (ab62378)

Overview

  • Product nameAnti-Pin1 (phospho S16) antibody [EP1480Y]
    See all Pin1 primary antibodies
  • Description
    Rabbit monoclonal [EP1480Y] to Pin1 (phospho S16)
  • SpecificityThis antibody detects Pin1 phosphorylated at Serine 16.
  • Tested applicationsSuitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Phospho specific peptide corresponding to residues surround Serine 16 of human Pin1.

  • Positive control
    • HeLa cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab62378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
ICC 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionEssential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzing pSer/Thr-Pro cis/trans isomerizations.
    • Sequence similaritiesContains 1 PpiC domain.
      Contains 1 WW domain.
    • DomainThe WW domain is required for the interaction with STIL and KIF20B.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • DOD antibody
      • DODO, Drosophila, homolog of antibody
      • FLJ40239 antibody
      • FLJ77628 antibody
      • MGC10717 antibody
      • NIMA interacting 1 antibody
      • Peptidyl prolyl cis trans isomerase NIMA interacting 1 antibody
      • Peptidyl prolyl cis/trans isomerase NIMA interacting antibody
      • Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 antibody
      • Peptidyl-prolyl cis-trans isomerase pin1 antibody
      • Peptidylprolyl cis/trans isomerase NIMA interacting 1 antibody
      • Pin 1 antibody
      • Pin1 antibody
      • PIN1_HUMAN antibody
      • PPIase Pin1 antibody
      • Prolyl isomerase antibody
      • Protein (peptidylprolyl cis/trans isomerase) NIMA interacting 1 antibody
      • Protein NIMA interacting 1 antibody
      • Rotamase Pin1 antibody
      • UBL 5 antibody
      • UBL5 antibody
      see all

    Anti-Pin1 (phospho S16) antibody [EP1480Y] images

    • All lanes : Anti-Pin1 (phospho S16) antibody [EP1480Y] (ab62378) at 1/500 dilution

      Lane 1 : HeLa cell lysate (untreated)
      Lane 2 : HeLa cell lysate (treated with FBS + Calyculin A)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP conjugated at 1/2000 dilution

      Predicted band size : 18 kDa
      Observed band size : 18 kDa

    References for Anti-Pin1 (phospho S16) antibody [EP1480Y] (ab62378)

    ab62378 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Liver)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
    Permeabilization Yes - Wash Buffer from Dako with Tween
    Specification Liver
    Fixative Paraformaldehyde
    Username

    Mr. Rudolf Jung

    Verified customer

    Submitted Oct 20 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"