Overview

  • Product nameAnti-PIN4 antibody
    See all PIN4 primary antibodies
  • Description
    Rabbit polyclonal to PIN4
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MPMAGLLKGL VRQLERFSVQ QQASKMPPKG KSGSGKAGKG GAASGSDSAD) of Human PIN4 isoform 2 (NP_006214; UniProt ID Q9Y237 isoform 2) conjugated to KLH.

  • Positive control
    • 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab98130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 14 for isoform 1 , for isoform 2, 16 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionIsoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA.
    Isoform 2 binds to double-stranded DNA.
  • Tissue specificityIsoform 2 is much more stable than isoform 1 (at protein level). Ubiquitous. Isoform 1 and isoform 2 are expressed in kidney, liver, blood vessel, brain, mammary gland, skeletal muscle, small intestine and submandibularis. Isoform 1 transcripts are much more abundant than isoform 2 in each tissue analyzed.
  • Sequence similaritiesBelongs to the ppiC/parvulin rotamase family. PIN4 subfamily.
    Contains 1 PpiC domain.
  • DomainThe PPIase domain enhances mitochondrial targeting.
  • Post-translational
    modifications
    Phosphorylated. Isoform 1 phosphorylation occurs both in the nucleus and the cytoplasm. Isoform 1 phosphorylation at Ser-19 does not affect its PPIase activity but is required for nuclear localization, and the dephosphorylation is a prerequisite for the binding to DNA. The unphosphorylated isoform 1 associates with the pre-rRNP complexes in the nucleus.
    Isoform 2 is sumoylated by SUMO2 and SUMO3.
  • Cellular localizationMitochondrion. Mitochondrion matrix. Imported in a time- and membrane potential-dependent manner to the mitochondrial matrix, but without concomitant processing of the protein. Directed to mitochondria by a novel N-terminal domain that functions as non-cleavable mitochondrial targeting peptide and Nucleus > nucleolus. Cytoplasm > cytoskeleton > spindle. Cytoplasm. Colocalizes in the nucleolus during interphase and on the spindle apparatus during mitosis with NPM1.
  • Information by UniProt
  • Database links
  • Alternative names
    • EPVH antibody
    • Eukaryotic parvulin homolog antibody
    • hEPVH antibody
    • hPar14 antibody
    • hPar17 antibody
    • MGC138486 antibody
    • OTTHUMP00000023527 antibody
    • OTTHUMP00000217483 antibody
    • OTTHUMP00000217485 antibody
    • Par14 antibody
    • Par17 antibody
    • Parvulin 14 antibody
    • Parvulin-14 antibody
    • Parvulin-17 antibody
    • Peptidyl prolyl cis trans isomerase NIMA interacting 4 antibody
    • Peptidyl prolyl cis/trans isomerase EPVH antibody
    • Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 antibody
    • Peptidyl-prolyl cis-trans isomerase PIN4 antibody
    • Peptidyl-prolyl cis/trans isomerase EPVH antibody
    • PIN4 antibody
    • PIN4_HUMAN antibody
    • PPIase Pin4 antibody
    • Protein (peptidylprolyl cis/trans isomerase) NIMA interacting, 4 (parvulin) antibody
    • Rotamase Pin4 antibody
    see all

Anti-PIN4 antibody images

  • Anti-PIN4 antibody (ab98130) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Predicted band size : 14 for isoform 1 , for isoform 2, 16 kDa

References for Anti-PIN4 antibody (ab98130)

ab98130 has not yet been referenced specifically in any publications.

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Vorweg erstmal zur Abbildung auf unserem Datenblatt:
Die Bande unterhalb der 22 kDa soll die für PIN4 spezifische Bande sein. Einerseits können die post-translationalen Modifikationen, die für PIN4 bekannt ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"