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Recombinant full length PINK1 protein (Human).
Our Abpromise guarantee covers the use of ab75487 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 2 µg/ml.|
|WB||1/100 - 1/500. Predicted molecular weight: 63 kDa.|
Immunocytochemistical detection of PINK1 antibody (ab75487) on Human Cell (PC12 cell line) Specification PC12 cell line Fixative: Paraformaldehyde. Permeabilization: 0.3xTriton 100. Blocking step: 10% serum for 1 hour @ 4°C. Primary antibody incubated at 2 µg/ml for 24 hours @ 4°C. Secondary antibody: Alexa Fluor® 488 (1/1000). The submitted image shows PC12 cells stained with ab75487; DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab75487 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"