Anti-PINK1 antibody [38CT18.7] (ab75487)

Overview

  • Product nameAnti-PINK1 antibody [38CT18.7]See all PINK1 primary antibodies ...
  • Description
    Mouse monoclonal [38CT18.7] to PINK1
  • Tested applicationsICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length PINK1 protein (Human).

  • Positive control
    • 293 cell lysate transiently transfected with the PINK gene

Properties

Applications

Our Abpromise guarantee covers the use of ab75487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 2 µg/ml.
WB 1/100 - 1/500. Predicted molecular weight: 63 kDa.
ELISA 1/1000.

Target

  • FunctionProtects against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). It is necessary for PARK2 recruitement to dysfunctional mitochondria to initiate their degradation.
  • Tissue specificityHighly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development.
  • Involvement in diseaseDefects in PINK1 are the cause of Parkinson disease type 6 (PARK6) [MIM:605909]. A neurodegenerative disorder characterized by parkinsonian signs such as rigidity, resting tremor and bradykinesia. A subset of patients manifest additional symptoms including hyperreflexia, autonomic instability, dementia and psychiatric disturbances. Symptoms show diurnal fluctuation and can improve after sleep.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localizationMitochondrion outer membrane. Cytoplasm > cytosol.
  • Information by UniProt
  • Database links
  • Alternative names
    • BRPK antibody
    • FLJ27236 antibody
    • mitochondrial antibody
    • PARK 6 antibody
    • PARK6 antibody
    • Phosphatase and Tensin Homolog antibody
    • PINK 1 antibody
    • PINK1 antibody
    • PINK1_HUMAN antibody
    • Protein kinase BRPK antibody
    • PTEN induced putative kinase 1 antibody
    • PTEN induced putative kinase protein 1 antibody
    • PTEN-induced putative kinase protein 1 antibody
    • Serine/threonine kinase PINK1 mitochondrial antibody
    • Serine/threonine protein kinase PINK1 mitochondrial antibody
    • Serine/threonine-protein kinase PINK1 antibody
    see all

Anti-PINK1 antibody [38CT18.7] images

  • All lanes : Anti-PINK1 antibody [38CT18.7] (ab75487) at 1/1000 dilution

    Lane 1 : Non-transfected 293 cell lysate
    Lane 2 : 293 cell lysate transiently transfected with the PINK1 gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 63 kDa
    Observed band size : 63 kDa
    Additional bands at : ~50 kDa. We are unsure as to the identity of these extra bands.
  • Immunocytochemistical detection of PINK1 antibody (ab75487) on Human Cell (PC12 cell line) Specification PC12 cell line Fixative: Paraformaldehyde.  Permeabilization: 0.3xTriton 100. Blocking step: 10% serum for 1 hour @ 4°C. Primary antibody incubated at 2 µg/ml for 24 hours @ 4°C. Secondary antibody: Alexa Fluor® 488 (1/1000). The submitted image shows PC12 cells stained with ab75487; DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    See Abreview

References for Anti-PINK1 antibody [38CT18.7] (ab75487)

ab75487 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Rat Cell lysate - whole cell (h9c2)
Loading amount 30 µg
Specification h9c2
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted May 02 2013

Application Western blot
Sample Human Cell lysate - whole cell (hek293 cell overexpression humanPINK1)
Loading amount 30 µg
Specification hek293 cell overexpression humanPINK1
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted Oct 17 2012

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Application Western blot
Sample Mouse Tissue lysate - whole (mouse heart)
Loading amount 50 µg
Specification mouse heart
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted Dec 23 2011

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (mouse cardiomyocyte)
Specification mouse cardiomyocyte
Fixative Paraformaldehyde
Permeabilization Yes - 1% triton x100
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted Dec 20 2011

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (PC12 cell line)
Specification PC12 cell line
Fixative Paraformaldehyde
Permeabilization Yes - 0.1x PBS plus 0.3xTriton x
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Jun 21 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"