Directly conjugated antibodies for immunofluorescence

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Anti-PINK1 antibody [38CT18.7] (ab75487)

Overview

  • Product nameAnti-PINK1 antibody [38CT18.7]
    See all PINK1 primary antibodies
  • Description
    Mouse monoclonal [38CT18.7] to PINK1
  • Tested applicationsIHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein corresponding to Human PINK1.

  • Positive control
    • WB: A431 cell lysate and mouse brain tissue lysate. IHC-P: Human kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab75487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ICC/IF Use a concentration of 1 - 2 µg/ml.
WB 1/100 - 1/500. Predicted molecular weight: 63 kDa.

Target

  • FunctionProtects against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). It is necessary for PARK2 recruitement to dysfunctional mitochondria to initiate their degradation.
  • Tissue specificityHighly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development.
  • Involvement in diseaseDefects in PINK1 are the cause of Parkinson disease type 6 (PARK6) [MIM:605909]. A neurodegenerative disorder characterized by parkinsonian signs such as rigidity, resting tremor and bradykinesia. A subset of patients manifest additional symptoms including hyperreflexia, autonomic instability, dementia and psychiatric disturbances. Symptoms show diurnal fluctuation and can improve after sleep.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localizationMitochondrion outer membrane. Cytoplasm > cytosol.
  • Information by UniProt
  • Database links
  • Alternative names
    • BRPK antibody
    • FLJ27236 antibody
    • mitochondrial antibody
    • PARK 6 antibody
    • PARK6 antibody
    • Phosphatase and Tensin Homolog antibody
    • PINK 1 antibody
    • PINK1 antibody
    • PINK1_HUMAN antibody
    • Protein kinase BRPK antibody
    • PTEN induced putative kinase 1 antibody
    • PTEN induced putative kinase protein 1 antibody
    • PTEN-induced putative kinase protein 1 antibody
    • Serine/threonine kinase PINK1 mitochondrial antibody
    • Serine/threonine protein kinase PINK1 mitochondrial antibody
    • Serine/threonine-protein kinase PINK1 antibody
    see all

Anti-PINK1 antibody [38CT18.7] images

  • All lanes : Anti-PINK1 antibody [38CT18.7] (ab75487) at 1000

    Lane 1 : A431 cell lysate
    Lane 2 : Mouse brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 63 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling PINK1 with ab75487. A peroxidase-conjugated anti-mouse IgG was used as the secondary antibody, followed by DAB staining.

  • Immunocytochemistry/Immunofluorescence analysis of PC12 cells labelling PINK1 with ab75487. Cells were fixed with paraformaldehyde, permeabilization with 0.3X Triton X-100 and blocked with 10% serum for 1 hour at 4°C. Cells were incubated with the primary antibody at 2 µg/ml for 24 hours at 4°C. An Alexa Fluor® 488-conjugated anti-mouse (1/1000) was used as the secondary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    See Abreview

References for Anti-PINK1 antibody [38CT18.7] (ab75487)

ab75487 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (liver, hepatocytes)
Specification liver, hepatocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Jan 06 2015

Application Western blot
Sample Rat Cell lysate - whole cell (h9c2)
Loading amount 30 µg
Specification h9c2
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
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Submitted May 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (hek293 cell overexpression humanPINK1)
Loading amount 30 µg
Specification hek293 cell overexpression humanPINK1
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
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Verified customer

Submitted Oct 17 2012

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (mouse heart)
Loading amount 50 µg
Specification mouse heart
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
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Verified customer

Submitted Dec 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (mouse cardiomyocyte)
Specification mouse cardiomyocyte
Fixative Paraformaldehyde
Permeabilization Yes - 1% triton x100
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: rt°C
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Abcam user community

Verified customer

Submitted Dec 20 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (PC12 cell line)
Specification PC12 cell line
Fixative Paraformaldehyde
Permeabilization Yes - 0.1x PBS plus 0.3xTriton x
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
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Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Jun 21 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"