Overview

  • Product name
    Anti-PINK1 antibody [38CT18.7]
    See all PINK1 primary antibodies
  • Description
    Mouse monoclonal [38CT18.7] to PINK1
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein corresponding to Human PINK1.

  • Positive control
    • WB: A431 cell lysate and mouse brain tissue lysate. IHC-P: Human kidney tissue. ICC/IF: PC-12 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab75487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ICC/IF Use a concentration of 1 - 2 µg/ml.
WB 1/100 - 1/500. Predicted molecular weight: 63 kDa.

Target

  • Function
    Protects against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). It is necessary for PARK2 recruitement to dysfunctional mitochondria to initiate their degradation.
  • Tissue specificity
    Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development.
  • Involvement in disease
    Defects in PINK1 are the cause of Parkinson disease type 6 (PARK6) [MIM:605909]. A neurodegenerative disorder characterized by parkinsonian signs such as rigidity, resting tremor and bradykinesia. A subset of patients manifest additional symptoms including hyperreflexia, autonomic instability, dementia and psychiatric disturbances. Symptoms show diurnal fluctuation and can improve after sleep.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localization
    Mitochondrion outer membrane. Cytoplasm > cytosol.
  • Information by UniProt
  • Database links
  • Alternative names
    • BRPK antibody
    • FLJ27236 antibody
    • mitochondrial antibody
    • PARK 6 antibody
    • PARK6 antibody
    • Phosphatase and Tensin Homolog antibody
    • PINK 1 antibody
    • PINK1 antibody
    • PINK1_HUMAN antibody
    • Protein kinase BRPK antibody
    • PTEN induced putative kinase 1 antibody
    • PTEN induced putative kinase protein 1 antibody
    • PTEN-induced putative kinase protein 1 antibody
    • Serine/threonine kinase PINK1 mitochondrial antibody
    • Serine/threonine protein kinase PINK1 mitochondrial antibody
    • Serine/threonine-protein kinase PINK1 antibody
    see all

Anti-PINK1 antibody [38CT18.7] images

  • All lanes : Anti-PINK1 antibody [38CT18.7] (ab75487) at 1/1000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : Mouse brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated goat anti-mouse IgG H&L at 1/5000 dilution

    Predicted band size : 63 kDa
    Observed band size : 63 kDa

    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human stomach section, labelling PINK1 with ab75487 (1/25). An undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining. 

  • Immunohistochemical analysis of paraffin-embedded Human heart section, labelling PINK1 with ab75487 (1/25). An undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining. 

  • Immunocytochemistry/Immunofluorescence analysis of PC-12 cells labelling PINK1 (green) with ab75487 at a dilution of 1/25. An Alexa Fluor® 488-conjugated goat anti-mouse IgG was used as the secondary antibody (1/400). Cytoplasmic actin was counterstained with Alexa Fluor® 555-conjugated with Phalloidin (red).

  • Immunocytochemistry/Immunofluorescence analysis of PC12 cells labelling PINK1 with ab75487. Cells were fixed with paraformaldehyde, permeabilization with 0.3X Triton X-100 and blocked with 10% serum for 1 hour at 4°C. Cells were incubated with the primary antibody at 2 µg/ml for 24 hours at 4°C. An Alexa Fluor® 488-conjugated anti-mouse (1/1000) was used as the secondary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    See Abreview

References for Anti-PINK1 antibody [38CT18.7] (ab75487)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293T)
Permeabilization
Yes - 0.1% Triton X-100
Specification
HEK293T
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 14 2017

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (liver, hepatocytes)
Specification
liver, hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 06 2015

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse cardiomyocyte)
Specification
mouse cardiomyocyte
Fixative
Paraformaldehyde
Permeabilization
Yes - 1% triton x100
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted Dec 20 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (PC12 cell line)
Specification
PC12 cell line
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1x PBS plus 0.3xTriton x
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Jun 21 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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