General notesPrincipal Names – PINX1; PIN2-interacting protein 1; LPTL; LPTS; MGC8850; FLJ20565; 67-11-3 protein; hepatocellular carcinoma-related putative tumor suppressor.
Official Gene Symbol - PINX1 (interim symbol).
GenBank Accession Number – NP_060354.
LocusLink ID - 54984; 72400 (mouse).
Please note: We have been contacted by a researcher working with PINX1 to advise that recent research has shown that there are two splice variants of PINX1, one at 45 kDa (full lenth) and the other at 20 kDa (C-terminus truncated). This antibody detects a band at 37kDa in Jurkat Lysate. The researcher is currently testing this antibody to confirm that the band we find at 37 kDa is infact PINX1. We would be interested to hear back from any other researchers working with PINX1. 11 March, 2004.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWestern Blot – Approx 35kDa bands seen in HeLa lysate (predicted molecular weight: 37 kDa).
Recommended for use at 1-3µg/ml.
FunctionMicrotubule-binding protein essential for faithful chromosome segregation. Mediates TRF1 and TERT accumulation in nucleolus and enhances TRF1 binding to telomeres. Inhibits telomerase activity. May inhibit cell proliferation and act as tumor suppressor.
Tissue specificityUbiquitous; expressed at low levels. Not detectable in a number of hepatocarcinoma cell lines.
Sequence similaritiesBelongs to the PINX1 family. Contains 1 G-patch domain.
DomainThe TID (telomerase inhibiting domain) domain is sufficient to bind TERT and inhibit its activity. The TBM domain mediates interaction with TERF1.
Cellular localizationNucleus. Nucleus > nucleolus. Chromosome > telomere. Chromosome > centromere > kinetochore. Localizes in nucleoli, at telomere speckles and to the outer plate of kinetochores. Localization to the kinetochore is mediated by its central region and depends on NDC80 and CENPE.
ab2344 staining (2µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
References for Anti-PINX1 antibody (ab2344)
This product has been referenced in:
Herrmann G et al. Conserved interactions of the splicing factor Ntr1/Spp382 with proteins involved in DNA double-strand break repair and telomere metabolism. Nucleic Acids Res35:2321-32 (2007).
Read more (PubMed: 17389648) »