Overview

  • Product name
  • Description
    Goat polyclonal to PINX1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    C-ESYLQPKAKRHTE

    , corresponding to internal sequence amino acids 225-237 of Human PINX1 (NP_060354.4).

  • Positive control
    • A549, HepG2 and K562 cell lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab99112 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 37 kDa).

Target

  • Function
    Microtubule-binding protein essential for faithful chromosome segregation. Mediates TRF1 and TERT accumulation in nucleolus and enhances TRF1 binding to telomeres. Inhibits telomerase activity. May inhibit cell proliferation and act as tumor suppressor.
  • Tissue specificity
    Ubiquitous; expressed at low levels. Not detectable in a number of hepatocarcinoma cell lines.
  • Sequence similarities
    Belongs to the PINX1 family.
    Contains 1 G-patch domain.
  • Domain
    The TID (telomerase inhibiting domain) domain is sufficient to bind TERT and inhibit its activity.
    The TBM domain mediates interaction with TERF1.
  • Cellular localization
    Nucleus. Nucleus > nucleolus. Chromosome > telomere. Chromosome > centromere > kinetochore. Localizes in nucleoli, at telomere speckles and to the outer plate of kinetochores. Localization to the kinetochore is mediated by its central region and depends on NDC80 and CENPE.
  • Information by UniProt
  • Database links
  • Alternative names
    • 67-11-3 protein antibody
    • FLJ20565 antibody
    • Hepatocellular carcinoma-related putative tumor suppressor antibody
    • Liver-related putative tumor suppressor antibody
    • LPTL antibody
    • LPTS antibody
    • MGC8850 antibody
    • OTTHUMP00000224984 antibody
    • OTTHUMP00000224985 antibody
    • OTTHUMP00000224986 antibody
    • OTTHUMP00000225053 antibody
    • OTTHUMP00000225054 antibody
    • PIN2 interacting protein 1 antibody
    • Pin2-interacting protein X1 antibody
    • PIN2/TERF1-interacting telomerase inhibitor 1 antibody
    • PINX1 antibody
    • PINX1_HUMAN antibody
    • Protein 67-11-3 antibody
    • TRF1-interacting protein 1 antibody
    see all

Images

  • Anti-PINX1 antibody (ab99112) at 0.3 µg/ml + HepG2 cell lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 37 kDa

References

This product has been referenced in:
  • Chen YL  et al. The telomerase inhibitor Gno1p/PINX1 activates the helicase Prp43p during ribosome biogenesis. Nucleic Acids Res 42:7330-45 (2014). WB ; Human . Read more (PubMed: 24823796) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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