Tissue, cells or virus corresponding to Zebrafish Osteoblast Marker.
Brain, Gut, and Eye tissue.
This antibody clone is manufactured by Abcam.
It is thought that axons of pioneer neurons serve as a pathway for additional neurons that develop later in the embryo and project their axons to the appropriate target. In many systems pioneer neurons are eliminated by programmed cell-death and are not present in the adult.
Location : Fin hair cell, hindbrain horizontal cell, retinal cone cell, retinal inner plexiform layer and pioneer neuron.
Product was previously marketed under the MitoSciences sub-brand.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Immunocytochemistry/ Immunofluorescence - Anti-Pioneer neuron marker [ZNS-2] antibody (ab183372)This image is courtesy of an Abreview submitted by Dr Ryan MacDonald
ab183372 staining in 4 dpf zebrafish embryo retina sections by IHC-Fr. The tissue was fixed with paraformaldehyde and permeabilization was performed using 0.5% Triton X. Blocking of the sample was done with 5% BSA in PBS containing 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab183372 at 1/100 in blocking solution for 16h at 4°C. An alexa 647conjugated goat anti-mouse polyclonal antibody at 1/500 was used as the secondary antibody. Nuclei are stained in blue with DAPI. The IPL and OPL can be observed.