Anti-Pioneer neuron marker antibody [ZNS-2] (ab183372)

Overview

  • Product name
    Anti-Pioneer neuron marker antibody [ZNS-2]
  • Description
    Mouse monoclonal [ZNS-2] to Pioneer neuron marker
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Zebrafish
    Does not react with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Zebrafish Osteoblast Marker.

  • Positive control
    • Brain, Gut, and Eye tissue.
  • General notes

    This antibody clone is manufactured by Abcam.

    It is thought that axons of pioneer neurons serve as a pathway for additional neurons that develop later in the embryo and project their axons to the appropriate target. In many systems pioneer neurons are eliminated by programmed cell-death and are not present in the adult.

     

    Location : Fin hair cell, hindbrain horizontal cell, retinal cone cell, retinal inner plexiform layer and pioneer neuron.

     

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

Our Abpromise guarantee covers the use of ab183372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Images

  • ab183372 staining in 4 dpf zebrafish embryo retina sections by IHC-Fr. The tissue was fixed with paraformaldehyde and permeabilization was performed using 0.5% Triton X. Blocking of the sample was done with 5% BSA in PBS containing 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab183372 at 1/100 in blocking solution for 16h at 4°C. An alexa 647conjugated goat anti-mouse polyclonal antibody at 1/500 was used as the secondary antibody. Nuclei are stained in blue with DAPI. The IPL and OPL can be observed.

    See Abreview

References

ab183372 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Zebrafish Tissue sections (Retina, Inner plexiform layer Outer plexiform laye)
Specification
Retina, Inner plexiform layer Outer plexiform laye
Permeabilization
Yes - triton X
Fixative
Paraformaldehyde
Username

Dr. Ryan Macdonald

Verified customer

Submitted May 01 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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