Overview

  • Product name
  • Description
    Mouse monoclonal to PIP2
  • Specificity
    This antibody cross reacts with Phosphatidic acid (<5.0%).
  • Tested applications
    Suitable for: ICC/IFmore details
  • Immunogen

    Phosphatidylinositol-4,5-biphosphate (PIP2) from bovine spinal cord

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab11039 as a replacement.

Properties

Applications

Our Abpromise guarantee covers the use of ab2335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.

TBS buffer is ideal.

Target

  • Relevance
    Phosphatidylinositol 4,5-biphosphate (PIP2) is a membrane phospholipid that has been implicated in a variety of cellular processes, including synaptic vesicle recycling and signal transduction pathways. PLCD4 hydrolyzes PIP2 to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3).

Images

  • ab2335, staining PIP2 in non-polarized (left) and polarized (right) AX3 cells by Immunocytochemisry/ Immunofluorescence.

    Cells were fixed with formaldehyde, glutaraldehyde, and 0.01% Triton X-100 in PBS for 10 min. Samples were incubated with primary antibody at 1/100 dilution.

References

This product has been referenced in:
  • Wahl S  et al. The Disease Protein Tulp1 Is Essential for Periactive Zone Endocytosis in Photoreceptor Ribbon Synapses. J Neurosci 36:2473-93 (2016). Read more (PubMed: 26911694) »
  • Li J  et al. Actin dynamics is rapidly regulated by the PTEN and PIP2 signaling pathways leading to myocyte hypertrophy. Am J Physiol Heart Circ Physiol 307:H1618-25 (2014). Read more (PubMed: 25260617) »

See all 5 Publications for this product

Customer reviews and Q&As

Thank you for contacting Abcam

I have talked to the supplier of this antibody and I am sorry but they do not have any detailed clone information that I can share and cannot confirm that this antibody is the same as the one published in the pa...

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Thank you very much for your call last week, and I'm very sorry for the delay getting back to you.

I've just heard from the lab, and unfortunately ab2335 has not been tested for cross-reactivity with PIP3. The original clone was produced and ...

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Thank you for your enquiry. The cross reactivity of this antibody with other PtdIns is stated below. PIP2 100%; Phosphatidic acid <5.0%; PIP 0.1%; Phosphatidyl inositol <0.2%; Phosphatidyl choline <0.2%; Phosphatidyl serine <0.2%; Phosphatidyl...

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Thank you for your enquiry. No ab2335 is not clone 2C11. Please do not hesitate to contact me should you require further assistance.

To our knowledge, this antibody has yet to be tested in ICC. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a rewar...

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Thank you for your enquiry, Here are two publications citing the use of this antibody: RIA: Fukami, K., Takenawa, T. (1989) Quantitative Changes in Polysphophoinositides 1,2-diacyglycerol and Inositol 1,4,5-Trisphosphate by Platelet-Derived Growth...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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