Overview

  • Product nameAnti-PIP3E antibody
  • Description
    Rabbit polyclonal to PIP3E
  • Tested applicationsSuitable for: ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide: AGIHKALENS FVTSESGFLN SLSSDDTSSL SSNHDHLTVP DKPAGSKIMD , corresponding to internal sequence amino acids 251-301 of Human PIP3E

  • Positive control
    • 721B cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab80817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/62500.

WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionEnhances the promotion of guanine-nucleotide exchange by PSCD2 on ARF6 in a concentration-dependent manner.
  • Sequence similaritiesContains 1 PH domain.
  • Cellular localizationCytoplasm. Cell membrane. Translocated with PSCD2 to the plasma membrane upon epidermal growth factor (EGF) stimulation.
  • Information by UniProt
  • Database links
  • Alternative names
    • ICEF1_HUMAN antibody
    • Interaction protein for cytohesin exchange factors 1 antibody
    • Interactor protein for cytohesin exchange factors 1 antibody
    • Ipcef1 antibody
    • Phosphoinositide binding protein PIP3 E antibody
    • Phosphoinositide binding protein PIP3E antibody
    • Phosphoinositide-binding protein PIP3-E antibody
    • RP3402L9 antibody
    see all

Anti-PIP3E antibody images

  • Anti-PIP3E antibody (ab80817) at 1 µg/ml + 721B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 49 kDa
    Observed band size : 49 kDa
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.
  • ab80817 staining PIP3E in human LNCaP Androgen Dependent Prostate cells treated with EGF for 24 hours by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 5% BSA for 1 hour at 20°C. Samples were incubated with primary antibody (1/50 in PBS + 1% BSA) for 24 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal (1/250) was used as the secondary antibody.

References for Anti-PIP3E antibody (ab80817)

ab80817 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (24 hr EGF-treated LNCaP Androgen Dependent Prostat)
Specification 24 hr EGF-treated LNCaP Androgen Dependent Prostat
Fixative Paraformaldehyde
Permeabilization Yes - Triton X-100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Skyla Carney

Verified customer

Submitted Oct 19 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"