The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/250 - 1/500.
Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionParticipates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate. Mediates RAC1-dependent reorganization of actin filaments. Acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs. Contributes to the activation of PLD2.
Tissue specificityHighly expressed in heart, placenta, skeletal muscle, kidney and pancreas. Detected at lower levels in brain, lung and liver.
Sequence similaritiesContains 1 PIPK domain.
Cellular localizationCell membrane. Endomembrane system. Golgi apparatus > Golgi stack. Nucleus speckle. Associated with the plasma membrane and with internal membranes. Associated with Golgi stacks (By similarity). Detected on RAC1-induced plasma membrane ruffles, and on membrane ruffles induced by platelet-derived growth factor. Localizes to nuclear speckles and associates with TUT1 to regulate polyadenylation of selected mRNAs.