Overview

  • Product name
    Anti-PIP5K1 alpha antibody
    See all PIP5K1 alpha primary antibodies
  • Description
    Rabbit polyclonal to PIP5K1 alpha
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen, corresponding to amino acids 266-389 of Human PIP5K1 alpha (Q99755).

  • Positive control
    • Human tonsil tissue; RT-4 and U-251 MG cells lysate; Human cell line A-431

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate. Mediates RAC1-dependent reorganization of actin filaments. Acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs. Contributes to the activation of PLD2.
  • Tissue specificity
    Highly expressed in heart, placenta, skeletal muscle, kidney and pancreas. Detected at lower levels in brain, lung and liver.
  • Sequence similarities
    Contains 1 PIPK domain.
  • Cellular localization
    Cell membrane. Endomembrane system. Golgi apparatus > Golgi stack. Nucleus speckle. Associated with the plasma membrane and with internal membranes. Associated with Golgi stacks (By similarity). Detected on RAC1-induced plasma membrane ruffles, and on membrane ruffles induced by platelet-derived growth factor. Localizes to nuclear speckles and associates with TUT1 to regulate polyadenylation of selected mRNAs.
  • Information by UniProt
  • Database links
  • Alternative names
    • 68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha antibody
    • Phosphatidylinositol 4 phosphate 5 kinase type I alpha antibody
    • Phosphatidylinositol-4-phosphate 5-kinase type I alpha antibody
    • Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha antibody
    • PI4P5K Ia antibody
    • PI51A_HUMAN antibody
    • PIP5K A antibody
    • PIP5K1-alpha antibody
    • Pip5k1a antibody
    • PIP5KIalpha antibody
    • PtdIns(4)P-5-kinase 1 alpha antibody
    see all

Images

  • All lanes : Anti-PIP5K1 alpha antibody (ab122049) at 1/250 dilution

    Lane 1 : RT-4 cells lysate
    Lane 2 : U-251 MG cells lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate
  • ab122049, at 1/125, staining PIP5K1 alpha in paraffin embedded Human tonsil tissue by Immunohistochemistry.
  • ab122049, at 4 µg/ml, staining PIP5K1 alpha in Human cell line A-431 by Immunofluorescence.

References

ab122049 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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