Anti-PIP5K1 alpha antibody (ab5466)

Overview

  • Product nameAnti-PIP5K1 alpha antibody
    See all PIP5K1 alpha primary antibodies
  • Description
    Rabbit polyclonal to PIP5K1 alpha
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to amino acids 34-50 of Human PIP5K1 A.

  • Positive control
    • HeLa cell lysate.
  • General notes


    Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThis antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
  • Primary antibody notesProtein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 61 kDa (predicted molecular weight: 65 kDa).

Target

  • FunctionParticipates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate. Mediates RAC1-dependent reorganization of actin filaments. Acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs. Contributes to the activation of PLD2.
  • Tissue specificityHighly expressed in heart, placenta, skeletal muscle, kidney and pancreas. Detected at lower levels in brain, lung and liver.
  • Sequence similaritiesContains 1 PIPK domain.
  • Cellular localizationCell membrane. Endomembrane system. Golgi apparatus > Golgi stack. Nucleus speckle. Associated with the plasma membrane and with internal membranes. Associated with Golgi stacks (By similarity). Detected on RAC1-induced plasma membrane ruffles, and on membrane ruffles induced by platelet-derived growth factor. Localizes to nuclear speckles and associates with TUT1 to regulate polyadenylation of selected mRNAs.
  • Information by UniProt
  • Database links
  • Alternative names
    • 68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha antibody
    • Phosphatidylinositol 4 phosphate 5 kinase type I alpha antibody
    • Phosphatidylinositol-4-phosphate 5-kinase type I alpha antibody
    • Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha antibody
    • PI4P5K Ia antibody
    • PI51A_HUMAN antibody
    • PIP5K A antibody
    • PIP5K1-alpha antibody
    • Pip5k1a antibody
    • PIP5KIalpha antibody
    • PtdIns(4)P-5-kinase 1 alpha antibody
    see all

Anti-PIP5K1 alpha antibody images



  • Predicted band size : 65 kDa


    ab5466 at a 1/100 dilution staining approximately 61kDa band of PIP5K 1A in HeLa cell lysate by Western blot (ECL).

References for Anti-PIP5K1 alpha antibody (ab5466)

ab5466 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. As stated on the online datasheet for this antibody, the immunogen used was human PIP5K1 alpha. The antibody has not yet been tested in mouse. I hope this information helps, please do not hesitate to contact us if yo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"