Anti-PIP5K1 beta antibody (ab5468)

Overview

  • Product name
  • Description
    Rabbit polyclonal to PIP5K1 beta
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to amino acids 1-18 of Human PIP5K1 B.

  • Positive control
    • Human placenta cell lysate.
  • General notes


    Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
  • Primary antibody notes
    Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 61 kDa (predicted molecular weight: 64 kDa).

Target

  • Function
    Mediates RAC1-dependent reorganization of actin filaments (By similarity). Participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate.
  • Tissue specificity
    Detected in heart, pancreas, brain, kidney, skeletal muscle and lung.
  • Sequence similarities
    Contains 1 PIPK domain.
  • Cellular localization
    Endomembrane system. Associated with membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • MSS 4 antibody
    • MSS4 antibody
    • Phosphatidylinositol 4 phosphate 5 kinase type 1 beta antibody
    • Phosphatidylinositol-4-phosphate 5-kinase type I beta antibody
    • Phosphatidylinositol-4-phosphate 5-kinase type-1 beta antibody
    • PI4P5K 1 beta antibody
    • PI51B_HUMAN antibody
    • PIP5K1-beta antibody
    • PIP5K1B antibody
    • PIP5KIbeta antibody
    • Pipk 5 beta antibody
    • Pipk 5b antibody
    • Protein STM 7 antibody
    • Protein STM-7 antibody
    • PtdIns(4)P-5-kinase 1 beta antibody
    • STM 7 antibody
    • STM7 antibody
    • STM7 LSB antibody
    • Type I phosphatidylinositol 4 phosphate 5 kinase beta antibody
    • Type I phosphatidylinositol-4-phosphate 5-kinase beta antibody
    see all

Images



  • Predicted band size : 64 kDa


    ab5468 at a 1/100 dilution staining approximately 61kDa band of PIP5K1 B in human placenta cell lysate by Western blot (ECL).
  • ab5468 staining PIP5K1 beta in human breast carcinoma (BC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References

ab5468 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your enquiry. To our knowledge, this antibody has not yet been tested for cross-reactivity with mouse. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not wor...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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