Anti-PIP5K1 beta antibody (ab5468)

Overview

  • Product nameAnti-PIP5K1 beta antibody
    See all PIP5K1 beta primary antibodies
  • Description
    Rabbit polyclonal to PIP5K1 beta
  • Tested applicationsSuitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to amino acids 1-18 of Human PIP5K1 B.

  • Positive control
    • Human placenta cell lysate.
  • General notes


    Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThis antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
  • Primary antibody notesProtein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 61 kDa (predicted molecular weight: 64 kDa).

Target

  • FunctionMediates RAC1-dependent reorganization of actin filaments (By similarity). Participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate.
  • Tissue specificityDetected in heart, pancreas, brain, kidney, skeletal muscle and lung.
  • Sequence similaritiesContains 1 PIPK domain.
  • Cellular localizationEndomembrane system. Associated with membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • MSS 4 antibody
    • MSS4 antibody
    • Phosphatidylinositol 4 phosphate 5 kinase type 1 beta antibody
    • Phosphatidylinositol-4-phosphate 5-kinase type I beta antibody
    • Phosphatidylinositol-4-phosphate 5-kinase type-1 beta antibody
    • PI4P5K 1 beta antibody
    • PI51B_HUMAN antibody
    • PIP5K1-beta antibody
    • PIP5K1B antibody
    • PIP5KIbeta antibody
    • Pipk 5 beta antibody
    • Pipk 5b antibody
    • Protein STM 7 antibody
    • Protein STM-7 antibody
    • PtdIns(4)P-5-kinase 1 beta antibody
    • STM 7 antibody
    • STM7 antibody
    • STM7 LSB antibody
    • Type I phosphatidylinositol 4 phosphate 5 kinase beta antibody
    • Type I phosphatidylinositol-4-phosphate 5-kinase beta antibody
    see all

Anti-PIP5K1 beta antibody images



  • Predicted band size : 64 kDa


    ab5468 at a 1/100 dilution staining approximately 61kDa band of PIP5K1 B in human placenta cell lysate by Western blot (ECL).
  • ab5468 staining PIP5K1 beta in human breast carcinoma (BC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References for Anti-PIP5K1 beta antibody (ab5468)

ab5468 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. To our knowledge, this antibody has not yet been tested for cross-reactivity with mouse. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not wor...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"