Anti-PIP5K2 alpha antibody [3A3] (ab125107)

Overview

  • Product nameAnti-PIP5K2 alpha antibody [3A3]
    See all PIP5K2 alpha primary antibodies
  • Description
    Mouse monoclonal [3A3] to PIP5K2 alpha
  • Tested applicationsSuitable for: Sandwich ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 304-366 of Human PIP5K2 alpha with a 26kDa propietary tag (NP_005019).

  • Positive control
    • K562 cell lysate; Human Spleen tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab125107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 46 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionCatalyzes the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol-4,5-biphosphate.
  • Tissue specificityExpressed ubiquitously, with high levels in the brain. Present in most tissues, except notably skeletal muscle and small intestine.
  • Sequence similaritiesContains 1 PIPK domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1 phosphatidylinositol 4 phosphate 5 kinase 2 alpha antibody
    • 1 phosphatidylinositol 4 phosphate 5 kinase antibody
    • 1 phosphatidylinositol 4 phosphate kinase antibody
    • 1-phosphatidylinositol-5-phosphate 4-kinase 2-alpha antibody
    • 4 5 kinase B isoform antibody
    • 4 5 kinase C isoform antibody
    • 4 5 kinase isoform 2 alpha antibody
    • Diphosphoinositide kinase 2 alpha antibody
    • Diphosphoinositide kinase 2-alpha antibody
    • FLJ13267 antibody
    • Phosphatidylinositol 4 phosphate 5 kinase type 2 alpha antibody
    • Phosphatidylinositol 4 phosphate 5 kinase type II alpha antibody
    • Phosphatidylinositol-5-phosphate 4-kinase type II alpha antibody
    • Phosphatidylinositol-5-phosphate 4-kinase type-2 alpha antibody
    • PI(5)P 4-kinase type II alpha antibody
    • PI42A_HUMAN antibody
    • PI5P4KA antibody
    • PIP4K2A antibody
    • PIP4KII-alpha antibody
    • PIP5K2 antibody
    • PIP5K2A antibody
    • PIP5KII alpha antibody
    • PIP5KII-alpha antibody
    • PIP5KIIA antibody
    • PIP5KIII antibody
    • PIPK antibody
    • PtdIns antibody
    • PtdIns(4)P-5-kinase B isoform antibody
    • PtdIns(4)P-5-kinase C isoform antibody
    • PtdIns(5)P-4-kinase isoform 2-alpha antibody
    • Type II phosphatidylinositol 4 phosphate 5 kinase 53 K isoform antibody
    see all

Anti-PIP5K2 alpha antibody [3A3] images



  • Predicted band size : 46 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: PIP5K2 alpha knockout HAP1 cell lysate (20 µg)
    Lane 3: K562 cell lysate(20 µg)
    Lane 4: Jurkat cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab125107 observed at 50 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab125107 was shown to recognize PIP5K2 alpha when PIP5K2 alpha knockout samples were used, along with additional cross-reactive bands. Wild-type and PIP5K2 alpha knockout samples were subjected to SDS-PAGE. ab125107 and ab181602 (loading control to GAPDH) were diluted 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Anti-PIP5K2 alpha antibody [3A3] (ab125107) at 1/500 dilution + K562 cell lysate

    Predicted band size : 46 kDa
  • ab125107, at 5 µg/ml, staining PIP5K2 alpha in Formalin-fixed, Paraffin-embedded Human Spleen tissue by Immunohistochemistry.
  • Using ab125107 as a capture antibody, the detection limit for recombinant proprietary tagged PIP5K2 alpha is approximately 3ng/ml.

References for Anti-PIP5K2 alpha antibody [3A3] (ab125107)

ab125107 has not yet been referenced specifically in any publications.

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