Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab55597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCatalyzes the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol-4,5-biphosphate.
    • Tissue specificityExpressed ubiquitously, with high levels in the brain. Present in most tissues, except notably skeletal muscle and small intestine.
    • Sequence similaritiesContains 1 PIPK domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • 1 phosphatidylinositol 4 phosphate 5 kinase 2 alpha antibody
      • 1 phosphatidylinositol 4 phosphate 5 kinase antibody
      • 1 phosphatidylinositol 4 phosphate kinase antibody
      • 1-phosphatidylinositol-5-phosphate 4-kinase 2-alpha antibody
      • 4 5 kinase B isoform antibody
      • 4 5 kinase C isoform antibody
      • 4 5 kinase isoform 2 alpha antibody
      • Diphosphoinositide kinase 2 alpha antibody
      • Diphosphoinositide kinase 2-alpha antibody
      • FLJ13267 antibody
      • Phosphatidylinositol 4 phosphate 5 kinase type 2 alpha antibody
      • Phosphatidylinositol 4 phosphate 5 kinase type II alpha antibody
      • Phosphatidylinositol-5-phosphate 4-kinase type II alpha antibody
      • Phosphatidylinositol-5-phosphate 4-kinase type-2 alpha antibody
      • PI(5)P 4-kinase type II alpha antibody
      • PI42A_HUMAN antibody
      • PI5P4KA antibody
      • PIP4K2A antibody
      • PIP4KII-alpha antibody
      • PIP5K2 antibody
      • PIP5K2A antibody
      • PIP5KII alpha antibody
      • PIP5KII-alpha antibody
      • PIP5KIIA antibody
      • PIP5KIII antibody
      • PIPK antibody
      • PtdIns antibody
      • PtdIns(4)P-5-kinase B isoform antibody
      • PtdIns(4)P-5-kinase C isoform antibody
      • PtdIns(5)P-4-kinase isoform 2-alpha antibody
      • Type II phosphatidylinositol 4 phosphate 5 kinase 53 K isoform antibody
      see all

    Anti-PIP5K2 alpha antibody images



    • Predicted band size : 46 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: PIP5K2 alpha knockout HAP1 cell lysate (20 µg)
      Lane 3: K562 cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab55597 observed at 50 kDa. Red - loading control, ab181602, observed at 37 kDa.

      ab55597 was shown to recognize PIP5K2 alpha when PIP5K2 alpha knockout samples were used, along with additional cross-reactive bands. Wild-type and PIP5K2 alpha knockout samples were subjected to SDS-PAGE. ab55597 and ab181602 (loading control to GAPDH) were diluted at 1 μg/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.



    • Predicted band size : 46 kDa
      PIP5K2 alpha antibody (ab55597) at 1ug/lane + K-562 cell lysate at 25ug/lane.

    References for Anti-PIP5K2 alpha antibody (ab55597)

    ab55597 has not yet been referenced specifically in any publications.

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