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Rabbit polyclonal to PIP5K2 alpha - N-terminal
HumanPredicted to work with:
Synthetic peptide, corresponding to a region within amino acids 1-43 (MATPGNLGSS VLASKTKTKK KHFVAQKVKL FRASDPLLSV LMW) of Human PIP5K2 alpha (Uniprot ID: P48426).
- A431, H1299, HeLa and HepG2 whole cell lysate. Human thyroid cancer tissue. HeLa cells.
Keep as concentrated solution.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89% PBS, 10% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/3000. Predicted molecular weight: 46 kDa.
||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/100 - 1/1000.
Catalyzes the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol-4,5-biphosphate.
Expressed ubiquitously, with high levels in the brain. Present in most tissues, except notably skeletal muscle and small intestine.
Contains 1 PIPK domain.
Information by UniProt
- 1 phosphatidylinositol 4 phosphate 5 kinase 2 alpha antibody
- 1 phosphatidylinositol 4 phosphate 5 kinase antibody
- 1 phosphatidylinositol 4 phosphate kinase antibody
Western blot - Anti-PIP5K2 alpha antibody - N-terminal (ab154585)
All lanes : Anti-PIP5K2 alpha antibody - N-terminal (ab154585) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 46 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PIP5K2 alpha antibody - N-terminal (ab154585)
Immunofluorescent analysis of methanol-fixed HeLa cells labeling PIP5K2 alpha with ab154585 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIP5K2 alpha antibody - N-terminal (ab154585)
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling PIP5K2 alpha with ab154585 at 1/100 dilution.
has not yet been referenced specifically in any publications.
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