Anti-PIP5K3 (phospho S307) antibody (ab62467)


  • Product name
    Anti-PIP5K3 (phospho S307) antibody
    See all PIP5K3 primary antibodies
  • Description
    Rabbit polyclonal to PIP5K3 (phospho S307)
  • Specificity
    ab62467 detects endogenous levels of PIP5K3 only when phosphorylated at serine 307.
  • Tested applications
    Suitable for: ELISA, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human PIP5K3 around the phosphorylation site of serine 307 (S-A-SP-I-T).

  • Positive control
    • Human brain tissue and HeLa cells.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab62467 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab62467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
ICC/IF 1/500 - 1/1000.
IHC-P 1/50 - 1/100.


  • Function
    The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 3,5-bisphosphate. Required for endocytic-vacuolar pathway and nuclear migration. Plays a role in the biogenesis of endosome carrier vesicles (ECV)/ multivesicular bodies (MVB) transport intermediates from early endosomes.
  • Involvement in disease
    Corneal dystrophy, fleck
  • Sequence similarities
    Contains 1 DEP domain.
    Contains 1 FYVE-type zinc finger.
    Contains 1 PIPK domain.
  • Cellular localization
    Endosome membrane. Mainly associated with membranes of the late endocytic pathway.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1 phosphatidylinositol 3 phosphate 5 kinase antibody
    • 1 phosphatidylinositol 4 phosphate 5 kinase antibody
    • 1-phosphatidylinositol 3-phosphate 5-kinase antibody
    • CFD antibody
    • epididymis luminal protein 37 antibody
    • FAB1 antibody
    • FAB1, S. cerevisiae, homolog of antibody
    • FYV1_HUMAN antibody
    • FYVE finger containing phosphoinositide kinase antibody
    • FYVE finger-containing phosphoinositide kinase antibody
    • KIAA0981 antibody
    • MGC40423 antibody
    • p235 antibody
    • Phosphatidylinositol 3 phosphate 5 kinase type III antibody
    • Phosphatidylinositol 3 phosphate/phosphatidylinositol 5 kinase type III antibody
    • Phosphatidylinositol 3-phosphate 5-kinase antibody
    • Phosphatidylinositol 3-phosphate 5-kinase type III antibody
    • Phosphatidylinositol 4 phosphate 5 kinase type III antibody
    • Phosphoinositide kinase, FYVE finger containing antibody
    • PIKfyve antibody
    • PIP5K antibody
    • PIP5K3 antibody
    • PIPkin III antibody
    • PIPkin-III antibody
    • PtdIns(4)P 5 kinase antibody
    • Type III PIP kinase antibody
    • ZFYVE29 antibody
    • Zinc finger, FYVE domain containing 29 antibody
    see all

Anti-PIP5K3 (phospho S307) antibody images

  • ab62467, at 1/50 dilution, staining PIP5K3 in paraffin embedded human brain tissue by Immunohistochemsitry in the absence (left image) or presence (right image) of the immunising peptide.
  • ab62467, at 1/500 dilution, staining PIP5K3 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.

References for Anti-PIP5K3 (phospho S307) antibody (ab62467)

ab62467 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (Mammary Epithelial)
Loading amount
50 µg
Mammary Epithelial
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Blocking step
Odyssey Blocking Buffer (Li-COR) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 30%

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Submitted Jul 08 2011


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