Recombinant
RabMAb

Anti-PIP5KI gamma antibody [MAO-R1] (ab109192)

Overview

  • Product name
    Anti-PIP5KI gamma antibody [MAO-R1]
    See all PIP5KI gamma primary antibodies
  • Description
    Rabbit monoclonal [MAO-R1] to PIP5KI gamma
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length Mouse PIP5KI gamma.

  • Positive control
    • K562, Human fetal kidney, C6, Neuro-2a, and 293T cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    MAO-R1
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109192 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 80-90 kDa (predicted molecular weight: 73 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function
      Plays a role in membrane ruffling and assembly of clathrin-coated pits at the synapse. Mediates RAC1-dependent reorganization of actin filaments (By similarity). Participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate.
    • Involvement in disease
      Defects in PIP5K1C are the cause of lethal congenital contracture syndrome type 3 (LCCS3) [MIM:611369]; also known as multiple contractural syndrome Israeli Bedouin type B. LCCS is an autosomal recessive disorder characterized by early fetal hydrops and akinesia, the Pena-Shokeir phenotype, specific neuropathology with degeneration of anterior horn neurons and extreme skeletal muscle atrophy. LCCS3 patients present at birth with severe multiple joint contractures with severe muscle wasting and atrophy, mainly in the legs. LCCS3 can be distinguished from the original LCCS by the absence of hydrops, fractures, and multiple pterygia.
    • Sequence similarities
      Contains 1 PIPK domain.
    • Cellular localization
      Cell membrane. Cytoplasmic, associated with the plasma membrane. Detected in focal adhesion plaques, membrane ruffles and plasma membrane invaginations.
    • Information by UniProt
    • Database links
    • Alternative names
      • Diphosphoinositide kinase antibody
      • LCCS3 antibody
      • Phosphatidylinositol-4-phosphate 5-kinase type I gamma antibody
      • Phosphatidylinositol-4-phosphate 5-kinase type-1 gamma antibody
      • PI51C antibody
      • PI51C_HUMAN antibody
      • PIP5K GAMMA antibody
      • PIP5K1-gamma antibody
      • Pip5k1c antibody
      • PIP5KIgamma antibody
      • PtdIns(4)P 5 kinase gamma antibody
      • PtdIns(4)P-5-kinase 1 gamma antibody
      • Type I PIP kinase antibody
      see all

    Images

    • All lanes : Anti-PIP5KI gamma antibody [MAO-R1] (ab109192) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : Human fetal kidney cell lysate
      Lane 3 : C6 cell lysate
      Lane 4 : Neuro-2a cell lysate
      Lane 5 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 73 kDa

    References

    This product has been referenced in:
    • Tojima T  et al. Steering neuronal growth cones by shifting the imbalance between exocytosis and endocytosis. J Neurosci 34:7165-78 (2014). Read more (PubMed: 24849351) »
    • Chen C  et al. Integrin a9ß1 in airway smooth muscle suppresses exaggerated airway narrowing. J Clin Invest 122:2916-27 (2012). Read more (PubMed: 22772469) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Thank you for contacting us and for your interest in ab109192. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products. We are happy to reassure our customers that all of our products are covered b...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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