Anti-PIP5KL1 antibody - N-terminal (ab170177)

Overview

  • Product name
    Anti-PIP5KL1 antibody - N-terminal
    See all PIP5KL1 primary antibodies
  • Description
    Rabbit polyclonal to PIP5KL1 - N-terminal
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PIP5KL1 aa 64-94 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q5T9C9

  • Positive control
    • K562 cell lysate; Human skeletal muscle tissue; U-251MG cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab170177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 45 kDa.
ICC/IF 1/10 - 1/50.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    May act as a scaffold to localize and regulate type I PI(4)P 5-kinases to specific compartments within the cell, where they generate PI(4,5)P2 for actin nucleation, signaling and scaffold protein recruitment and conversion to PI(3,4,5)P3.
  • Sequence similarities
    Contains 1 PIPK domain.
  • Cellular localization
    Cytoplasm. Membrane. Localized to large cytoplasmic vesicular structures.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA203J24.5 antibody
    • EC 2.7.1.68 antibody
    • MGC46424 antibody
    • phosphatidylinositol 4 phosphate 5 kinase like 1 antibody
    • phosphatidylinositol phosphate kinase homolog antibody
    • Phosphatidylinositol-4-phosphate 5-kinase-like protein 1 antibody
    • PI(4)P 5 kinase like protein 1 antibody
    • PI(4)P 5-kinase-like protein 1 antibody
    • PI5L1_HUMAN antibody
    • PIP5KL1 antibody
    • PIPKH antibody
    • PtdIns(4)P 5 kinase like protein 1 antibody
    • PtdIns(4)P-5-kinase-like protein 1 antibody
    • RP11-203J24.5 antibody
    see all

Images

  • Confocal immunofluorescent analysis of U-251MG cells labeling PIP5KL1 with ab170177 at 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclei (blue).

  • Anti-PIP5KL1 antibody - N-terminal (ab170177) at 1/100 dilution + K562 cell lysate at 35 µg

    Predicted band size : 45 kDa

References

ab170177 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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