Overview

  • Product nameAnti-Pirin antibody
    See all Pirin primary antibodies
  • Description
    Goat polyclonal to Pirin
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    GSSKKVTLSVLSREC

    , corresponding to N terminal amino acids 2-15 of Human Pirin.

  • Positive control
    • Human heart lysate. Lysate prepared from transfected HEK293 transiently expressing Pirin.

Properties

Applications

Our Abpromise guarantee covers the use of ab21202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 30-35 kDa (predicted molecular weight: 32 kDa).

Target

  • FunctionPossible transcriptional coregulator. May contribute to the regulation of cellular processes via its interaction with BCL3. May be required for efficient terminal myeloid maturation of hematopoietic cells. May play a role in the regulation of cell migration. May promote apoptosis when overexpressed. Has quercetin 2,3-dioxygenase activity (in vitro).
  • Tissue specificityHighly expressed in a subset of melanomas. Detected at very low levels in most tissues (at protein level). Expressed in all tissues, with highest level of expression in heart and liver.
  • PathwayFlavonoid metabolism; quercetin degradation.
  • Sequence similaritiesBelongs to the pirin family.
  • Cellular localizationNucleus. Cytoplasm. Predominantly localized in dot-like subnuclear structures. Cytoplasmic localization of PIR seems to positively correlate with melanoma progression.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3-dioxygenase PIR antibody
    • Iron binding nuclear protein antibody
    • Pir antibody
    • PIR_HUMAN antibody
    • Pirin antibody
    • Probable quercetin 2 antibody
    • probable quercetin 2,3-dioxygenase PIR antibody
    • Probable quercetinase antibody
    see all

Anti-Pirin antibody images

  • Anti-Pirin antibody (ab21202) at 0.3 µg/ml + Human Heart lysate (35µg protein in RIPA buffer).
    Developed using the ECL technique

    Predicted band size : 32 kDa
    Observed band size : 30-35 kDa (why is the actual band size different from the predicted?)
  • Anti-Pirin antibody (ab21202) at 1 µg/ml + lysate prepared from transfected HEK293 cells transiently over-expressing Pirin.

    Predicted band size : 32 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 32 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Pirin antibody (ab21202)

This product has been referenced in:
  • Brzóska K  et al. Basal PIR expression in HeLa cells is driven by NRF2 via evolutionary conserved antioxidant response element. Mol Cell Biochem 389:99-111 (2014). WB ; Human . Read more (PubMed: 24390086) »

See 1 Publication for this product

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