Overview

  • Product name
  • Description
    Rabbit polyclonal to Pirin
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide: GEPLREPVIQ HGPFVMNTNE EISQAILDFR NAKNGFERAK TWKSKIGN, corresponding to amino acids 243-290 of Human Pirin

  • Positive control
    • HepG2 cell lystes and Human lung tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab55978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Possible transcriptional coregulator. May contribute to the regulation of cellular processes via its interaction with BCL3. May be required for efficient terminal myeloid maturation of hematopoietic cells. May play a role in the regulation of cell migration. May promote apoptosis when overexpressed. Has quercetin 2,3-dioxygenase activity (in vitro).
  • Tissue specificity
    Highly expressed in a subset of melanomas. Detected at very low levels in most tissues (at protein level). Expressed in all tissues, with highest level of expression in heart and liver.
  • Pathway
    Flavonoid metabolism; quercetin degradation.
  • Sequence similarities
    Belongs to the pirin family.
  • Cellular localization
    Nucleus. Cytoplasm. Predominantly localized in dot-like subnuclear structures. Cytoplasmic localization of PIR seems to positively correlate with melanoma progression.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3-dioxygenase PIR antibody
    • Iron binding nuclear protein antibody
    • Pir antibody
    • PIR_HUMAN antibody
    • Pirin antibody
    • Probable quercetin 2 antibody
    • probable quercetin 2,3-dioxygenase PIR antibody
    • Probable quercetinase antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling Pirin with ab55978 at 4-8µg/ml. Arrow indicate postively labelled alveolar cells. Magnification: 400X.

  • Anti-Pirin antibody (ab55978) at 1.25 µg/ml + HepG2 cell lyste at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 32 kDa
    Observed band size : 32 kDa

References

ab55978 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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