Overview

  • Product name
  • Description
    Rabbit polyclonal to PIST
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 275-325 (DSLKKSQGVG PIRKVLLLKE DHEGLGISIT GGKEHGVPIL ISEIHPGQPA D), of Human PIST (NP_065132.1).

  • Positive control
    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab95067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Plays a role in intracellular protein trafficking and degradation. May regulate CFTR chloride currents and acid-induced ACCN3 currents by modulating cell surface expression of both channels. May also regulate the intracellular trafficking of the ADR1B receptor. May play a role in autophagy. Overexpression results in CFTR intracellular retention and degradation in the lysosomes.
    • Tissue specificity
      Ubiquitously expressed.
    • Involvement in disease
      Note=A chromosomal aberration involving GOPC is found in a glioblastoma multiforme sample. An intra-chromosomal deletion del(6)(q21q21) is responsible for the formation of GOPC-ROS1 chimeric protein which has a constitutive receptor tyrosine kinase activity.
    • Sequence similarities
      Contains 1 PDZ (DHR) domain.
    • Domain
      The PDZ domain mediates interactions with FZD5, FZD8, ACCN3, GRID2, CLCN3 (By similarity). Mediates also interaction with CFTR and ADRB1.
      The coiled-coil region probably mediates association to membranes, targeting to the Golgi, and interactions with GOLGA3, and STX6. May also mediate interaction with RHOQ.
    • Cellular localization
      Cytoplasm. Golgi apparatus membrane. Golgi apparatus > trans-Golgi network membrane. Cell junction > synapse. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell projection > dendrite. Enriched in synaptosomal and postsynaptic densities (PSD) fractions (By similarity). Expressed in cell bodies and dendrites of Purkinje cells (By similarity). Localized at the trans-Golgi network (TGN) of spermatids and the medulla of round spermatides.
    • Information by UniProt
    • Database links
    • Alternative names
      • CAL antibody
      • CFTR associated ligand antibody
      • CFTR-associated ligand antibody
      • dJ94G16.2 antibody
      • dJ94G16.2 PIST antibody
      • FIG antibody
      • Fused in glioblastoma antibody
      • Golgi associated PDZ and coiled coil motif containing antibody
      • Golgi associated PDZ and coiled coil motif containing protein antibody
      • Golgi-associated PDZ and coiled-coil motif-containing protein antibody
      • GOPC 1 antibody
      • GOPC antibody
      • GOPC_HUMAN antibody
      • GOPC1 antibody
      • OTTHUMP00000040403 antibody
      • PDZ protein interacting specifically with TC 10 antibody
      • PDZ protein interacting specifically with TC10 antibody
      • PDZ/coiled coil domain binding partner for the rho family GTPase TC 10 antibody
      • PDZ/coiled coil domain binding partner for the rho family GTPase TC10 antibody
      • PIST antibody
      • Protein interacting specifically with Tc 10 antibody
      • Protein interacting specifically with Tc10 antibody
      see all

    Images

    • ab95067 detecting PIST in HeLa whole cell lysate by WB following IP.
      Lane 1: ab95067 at 3 µg/mg of lysate
      Lane 2: IP with an antibody which recognizes a downstream epitope of PIST
      Lane 3: control IgG.
      In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
      Detection: Chemiluminescence with an exposure time of 3 seconds.

    References

    ab95067 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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