Anti-PIST antibody [EPR4079] (ab109119)

Overview

  • Product nameAnti-PIST antibody [EPR4079]
    See all PIST primary antibodies
  • Description
    Rabbit monoclonal [EPR4079] to PIST
  • Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • WB: A375, 293T, and PC-3 cell lysates and human prostate and fetal kidney tissue lysates. IHC-P: Human stomach tissue. ICC/IF: HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab109119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 51 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • FunctionPlays a role in intracellular protein trafficking and degradation. May regulate CFTR chloride currents and acid-induced ACCN3 currents by modulating cell surface expression of both channels. May also regulate the intracellular trafficking of the ADR1B receptor. May play a role in autophagy. Overexpression results in CFTR intracellular retention and degradation in the lysosomes.
  • Tissue specificityUbiquitously expressed.
  • Involvement in diseaseNote=A chromosomal aberration involving GOPC is found in a glioblastoma multiforme sample. An intra-chromosomal deletion del(6)(q21q21) is responsible for the formation of GOPC-ROS1 chimeric protein which has a constitutive receptor tyrosine kinase activity.
  • Sequence similaritiesContains 1 PDZ (DHR) domain.
  • DomainThe PDZ domain mediates interactions with FZD5, FZD8, ACCN3, GRID2, CLCN3 (By similarity). Mediates also interaction with CFTR and ADRB1.
    The coiled-coil region probably mediates association to membranes, targeting to the Golgi, and interactions with GOLGA3, and STX6. May also mediate interaction with RHOQ.
  • Cellular localizationCytoplasm. Golgi apparatus membrane. Golgi apparatus > trans-Golgi network membrane. Cell junction > synapse. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell projection > dendrite. Enriched in synaptosomal and postsynaptic densities (PSD) fractions (By similarity). Expressed in cell bodies and dendrites of Purkinje cells (By similarity). Localized at the trans-Golgi network (TGN) of spermatids and the medulla of round spermatides.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAL antibody
    • CFTR associated ligand antibody
    • CFTR-associated ligand antibody
    • dJ94G16.2 antibody
    • dJ94G16.2 PIST antibody
    • FIG antibody
    • Fused in glioblastoma antibody
    • Golgi associated PDZ and coiled coil motif containing antibody
    • Golgi associated PDZ and coiled coil motif containing protein antibody
    • Golgi-associated PDZ and coiled-coil motif-containing protein antibody
    • GOPC 1 antibody
    • GOPC antibody
    • GOPC_HUMAN antibody
    • GOPC1 antibody
    • OTTHUMP00000040403 antibody
    • PDZ protein interacting specifically with TC 10 antibody
    • PDZ protein interacting specifically with TC10 antibody
    • PDZ/coiled coil domain binding partner for the rho family GTPase TC 10 antibody
    • PDZ/coiled coil domain binding partner for the rho family GTPase TC10 antibody
    • PIST antibody
    • Protein interacting specifically with Tc 10 antibody
    • Protein interacting specifically with Tc10 antibody
    see all

Anti-PIST antibody [EPR4079] images

  • ab109119 stained HeLa cells. The cells were 4% formaldhyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109119 at 1/100) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-PIST antibody [EPR4079] (ab109119) at 1/1000 dilution

    Lane 1 : A375 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Fetal kidney lysate
    Lane 4 : PC-3 cell lysate
    Lane 5 : Human prostate lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 51 kDa
  • Immunohistochemical staining of PIST expression in paraffin-embedded Human stomach tissue using ab109119 at 1/250 dilution.
  • ab109119 showing positive staining in Normal brain tissue.

  • ab109119 showing positive staining in Normal breast tissue.

  • ab109119 showing positive staining in Normal kidney tissue.

  • ab109119 showing positive staining in Normal liver tissue.

  • ab109119 showing positive staining in Normal ovary tissue.

References for Anti-PIST antibody [EPR4079] (ab109119)

ab109119 has not yet been referenced specifically in any publications.

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PIST has three isoforms: isoform 1 at 50.5 kDa and isoform 2 at 49.7 kDa (likely to appear as either a single thick band or a doublet), as well as isoform 3 at 35 kDa. These isoforms may be differentially expresse...

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