Overview

  • Product nameAnti-Pit1 antibody
    See all Pit1 primary antibodies
  • Description
    Rabbit polyclonal to Pit1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 216-265 (KRRTTISIAA KDALERHFGE QNKPSSQEIM RMAEELNLEK EVVRVWFCNR) of Human Pit1 (NP_000297).

  • Positive control
    • Human fetal liver lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab84803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionTranscription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3'.
  • Involvement in diseaseDefects in POU1F1 are the cause of pituitary hormone deficiency combined type 1 (CPHD1) [MIM:613038]. CPHD is characterized by impaired production of growth hormone (GH) and one or more of the other five anterior pituitary hormones.
  • Sequence similaritiesBelongs to the POU transcription factor family. Class-1 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dwarf antibody
    • GHF 1 antibody
    • GHF 1A antibody
    • GHF-1 antibody
    • GHF1 antibody
    • GHF1A antibody
    • Growth hormone factor 1 antibody
    • Hmp 1 antibody
    • Hmp1 antibody
    • Pit 1 antibody
    • Pit 1 beta antibody
    • PIT 1Z antibody
    • PiT-1 antibody
    • Pit1 beta antibody
    • PIT1_HUMAN antibody
    • PIT1Z antibody
    • Pituitary growth hormone antibody
    • Pituitary specific positive transcription factor 1 antibody
    • Pituitary-specific positive transcription factor 1 antibody
    • POU class 1 homeobox 1 antibody
    • POU domain class 1 transcription factor 1 antibody
    • POU domain transcriptional regulator antibody
    • POU1F1 antibody
    see all

Anti-Pit1 antibody images

  • Anti-Pit1 antibody (ab84803) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 33 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)

References for Anti-Pit1 antibody (ab84803)

ab84803 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"