Overview

  • Product name
  • Description
    Rabbit polyclonal to PITRM1
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-188 of Human PITRM1 (AAH01150).

  • Positive control
    • A549, HeLa, HepG2 and HCT116 cells and whole cell lysates; PC13 xenograft tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab111681 is purified by antigen-affinity chromatography.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab111681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 117 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

Target

  • Function
    ATP-independent protease that degrades mitochondrial transit peptides after their cleavage. Also degrades other unstructured peptides. Specific for peptides in the range of 10 to 65 residues. Able to degrade amyloid beta A4 (APP) protein when it accumulates in mitochondrion, suggesting a link with Alzheimer disease. Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference.
  • Tissue specificity
    Widely expressed. Expressed at higher level in muscle and heart compared to brain, pancreas, liver, lung and placenta.
  • Sequence similarities
    Belongs to the peptidase M16 family. PreP subfamily.
  • Post-translational
    modifications
    The disulfide bond may lock the enzyme in a closed conformation under oxidized conditions, suggesting that it may participate in redox regulation of the enzyme.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • hMP1 antibody
    • hPreP antibody
    • KIAA1104 antibody
    • Metalloprotease 1 antibody
    • MGC138192 antibody
    • MGC141929 antibody
    • mitochondrial antibody
    • MP1 antibody
    • Pitrilysin metallopeptidase 1 antibody
    • Pitrilysin metalloproteinase 1 antibody
    • PITRM 1 antibody
    • PITRM1 antibody
    • PreP antibody
    • PreP peptidasome antibody
    • PREP_HUMAN antibody
    • Presequence protease antibody
    • Presequence protease mitochondrial antibody
    see all

Anti-PITRM1 antibody images

  • Anti-PITRM1 antibody (ab111681) at 1/1000 dilution + A549 whole cell lysate at 30 µg

    Predicted band size : 117 kDa
  • ab111681 at 1/200 dilution staining PITRM1 in methanol-fixed HeLa cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.
  • ab111681 at 1/500 dilution staining PITRM1 in paraffin-embedded PC13 xenograft tissue by Immunohistochemistry.

References for Anti-PITRM1 antibody (ab111681)

ab111681 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab111681.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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