The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 117 kDa).
Use at an assay dependent dilution.
FunctionATP-independent protease that degrades mitochondrial transit peptides after their cleavage. Also degrades other unstructured peptides. Specific for peptides in the range of 10 to 65 residues. Able to degrade amyloid beta A4 (APP) protein when it accumulates in mitochondrion, suggesting a link with Alzheimer disease. Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference.
Tissue specificityWidely expressed. Expressed at higher level in muscle and heart compared to brain, pancreas, liver, lung and placenta.
Sequence similaritiesBelongs to the peptidase M16 family. PreP subfamily.
Post-translational modificationsThe disulfide bond may lock the enzyme in a closed conformation under oxidized conditions, suggesting that it may participate in redox regulation of the enzyme.