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Product name
Pitstop 2™
Description
Novel, selective cell permeable clathrin inhibitor
Biological description
Novel, selective, cell membrane permeable clathrin inhibitor. Competitively inhibits clathrin terminal domain to selectively inhibit clathrin mediated endocytosis (CME) (IC50 = 12 μM for inhibition of amphiphysin association of clathrin TD). Interferes with receptor mediated endocytosis (RME), entry of HIV and synaptic vesicle recycling.
General notes
A stock solution should be prepared by dissolving in 100% DMSO (fresh, sterile) to give a final concentration of 30 mM. Vortexing should be used to ensure solublization of the compound. This stock solution is stable at room temperature for about 4-6 hrs. Preparing initial stock solutions that are too low in concentration limits later use of the compound in aqueous buffers, as the DMSO concentration in the final aqueous solution should not exceed about 0.1%. Aliquots of the stock solution should be stored at -20 °C. Stock solutions should not be left at room temperature for longer than necessary to prepare the working solutions. Avoid freeze/thaw cycles of the stock solution as this may affect the activity of the compound. The compound will remain soluble in 1% DMSO. However, at concentrations of 300 μM or higher, precipitation may occur (depending on which aqueous buffer is used and the temperature).
See protocols below for full guidelines for use
Properties
Chemical name
N-[5-(4-Bromobenzylidene)-4-oxo-4,5-dihydro-1,3-thiazol-2-yl]naphthalene-1-sulfonamide
Molecular Weight
473.36
Chemical structure
Molecular formula
C20H13BrN2O3S2
Solubility
Soluble in DMSO
PubChem identifier
0
Storage instructions
Store at +4°C (desiccating conditions). Do not freeze.
Research areas
Biochemicals >> Research Area >> Stroke >> Signaling >> Endocytosis & exocytosis >> Clathrin
Biochemicals >> Research Area >> Respiratory disease >> Signaling >> Endocytosis & exocytosis >> Clathrin
Biochemicals >> Research Area >> Pain & inflammation >> Signaling >> Endocytosis & exocytosis >> Clathrin
Biochemicals >> Research Area >> Obesity >> Signaling >> Endocytosis & exocytosis >> Clathrin
Biochemicals >> Research Area >> Hypertension >> Signaling >> Endocytosis & exocytosis >> Clathrin
Biochemicals >> Research Area >> Heart disease >> Signaling >> Endocytosis & exocytosis >> Clathrin
Biochemicals >> Research Area >> Diabetes >> Signaling >> Endocytosis & exocytosis >> Clathrin
Biochemicals >> Research Area >> Cancer >> Signaling >> Endocytosis & exocytosis >> Clathrin
Biochemicals >> Research Area >> Alzheimer's Disease >> Signaling >> Endocytosis & exocytosis >> Clathrin
Biochemicals >> Pharmacology >> Signaling >> Endocytosis & exocytosis >> Clathrin
Biochemicals >> Chemical Type >> Biochemicals
Protocols
References for Pitstop 2™ (ab120687)
This product has been referenced in:
- von Kleist L et al. Role of the clathrin terminal domain in regulating coated pit dynamics revealed by small molecule inhibition. Cell 146:471-84 (2011).Read more (PubMed: 21816279) »
See 1 publication for this product
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"