- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-PITX1 antibody
See all PITX1 primary antibodies -
DescriptionRabbit polyclonal to PITX1
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Host speciesRabbit
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Tested applicationsSuitable for: ELISA, WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Dog
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Immunogen
A region within synthetic peptide: GTPASPYSVY RDTCNSSLAS LRLKSKQHSS FGYGALQGPA SGLNACQYNS, corresponding to C terminal amino acids 265-314 of Human PITX1
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Positive control
- Jurkat cell lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab66943 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. ELISA titre using peptide based assay: 1/1562500. |
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| WB | Use a concentration of 1.25 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
Target
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FunctionMay play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb.
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Involvement in diseaseDefects in PITX1 are a cause of congenital clubfoot (CCF) [MIM:119800]; also known as talipes equinovarus (TEV). Clubfoot is a congenital limb deformity defined as fixation of the foot in cavus, adductus, varus, and equinus (i.e. inclined inwards, axially rotated outwards, and pointing downwards) with concomitant soft tissue abnormalities. Clubfoot may occur in isolation or as part of a syndrome. Clubfoot appears to be a multifactorial trait.
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Sequence similaritiesBelongs to the paired homeobox family. Bicoid subfamily.
Contains 1 homeobox DNA-binding domain. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 374201 Chicken
- Entrez Gene: 5307 Human
- Entrez Gene: 18740 Mouse
- Entrez Gene: 113983 Rat
- Omim: 602149 Human
- SwissProt: P56673 Chicken
- SwissProt: P78337 Human
- SwissProt: P70314 Mouse
see all -
Alternative names
- BFT antibody
- CCF antibody
- Hindlimb expressed homeobox protein backfoot antibody
see all
Images
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Western blot - Anti-PITX1 antibody (ab66943)All lanes : Anti-PITX1 antibody (ab66943) at 1.25 µg/ml
Lane 1 : Marker
Lane 2 : Jurkat cell lysate at 10 µg
Secondary
All lanes : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 34 kDa
Observed band size: 37 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
Protocols
References
ab66943 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"