The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2500 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).
Use at 1-4 µg/mg of lysate.
May play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb.
Involvement in disease
Defects in PITX1 are a cause of congenital clubfoot (CCF) [MIM:119800]; also known as talipes equinovarus (TEV). Clubfoot is a congenital limb deformity defined as fixation of the foot in cavus, adductus, varus, and equinus (i.e. inclined inwards, axially rotated outwards, and pointing downwards) with concomitant soft tissue abnormalities. Clubfoot may occur in isolation or as part of a syndrome. Clubfoot appears to be a multifactorial trait.
Belongs to the paired homeobox family. Bicoid subfamily. Contains 1 homeobox DNA-binding domain.
HeLa whole cell lysate (1 mg) was immunoprecipitated using ab70273 at 3 µg/mg lysate (lane 1) or an IgG control (lane 2). One quarter of the total IP reaction was loaded on to a gel and analysed by Western blot using ab70273 at 1 µg/ml. Bands were detected using chemiluminescence with an exposure time of 30 seconds.
Western blot - PITX1 antibody (ab70273)
All lanes : Anti-PITX1 antibody (ab70273) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg