Anti-PITX2 antibody (ab32832)


  • Product nameAnti-PITX2 antibody
    See all PITX2 primary antibodies
  • Description
    Rabbit polyclonal to PITX2
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide: SVLAPGQPRS LDSSKHRLEV HTISDTSSPE AAEKDKSQQG KNEDVGAEDP, corresponding to a region within N terminal amino acids 37-86 of Human PITX2

  • Positive control
    • HepG2 cell lysate. Human intestine.


Associated products


Our Abpromise guarantee covers the use of ab32832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/62500.
WB Use a concentration of 2.5 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.


  • FunctionMay play an important role in development and maintenance of anterior structures. Isoform PTX2C is involved in left-right asymmetry the developing embryo.
  • Involvement in diseaseDefects in PITX2 are the cause of Axenfeld-Rieger syndrome type 1 (RIEG1) [MIM:180500]; also known as Rieger syndrome type 1. RIEG1 is an autosomal dominant defect characterized by hypodontia (partial anodontia), anal stenosis, hypertelorism, mental deficiency, agenesis of the facial bones, with malformation of the anterior chamber of the eye.
    Defects in PITX2 are the cause of iridogoniodysgenesis type 2 (IRID2) [MIM:137600]; also known as iridogoniodysgenesis syndrome 2 (IGDS2). It is an autosomal dominant inherited disease.
    Defects in PITX2 are a cause of Peters anomaly (PAN) [MIM:604229]. It is a congenital defect of the anterior chamber of the eye.
    Defects in PITX2 are associated with ring dermoid of cornea (RDC) [MIM:180550]. RDC is an autosomal dominantly inherited syndrome characterized by bilateral annular limbal dermoids with corneal and conjunctival extension.
  • Sequence similaritiesBelongs to the paired homeobox family. Bicoid subfamily.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • All1 responsive gene 1 antibody
    • ALL1 responsive protein ARP1 antibody
    • ALL1-responsive protein ARP1 antibody
    • ARP 1 antibody
    • ARP1 antibody
    • Brx 1 antibody
    • Brx1 antibody
    • Homeobox protein PITX2 antibody
    • IDG 2 antibody
    • IDG2 antibody
    • IGDS 2 antibody
    • IGDS antibody
    • IGDS2 antibody
    • IHG 2 antibody
    • IHG2 antibody
    • IRID 2 antibody
    • IRID2 antibody
    • MGC111022 antibody
    • MGC20144 antibody
    • Otlx 2 antibody
    • Otlx2 antibody
    • Paired like homeodomain transcription factor 2 antibody
    • Paired-like homeodomain transcription factor 2 antibody
    • Pituitary homeo box 2 antibody
    • Pituitary homeobox 2 antibody
    • PITX 2 antibody
    • pitx2 antibody
    • PITX2_HUMAN antibody
    • PTX 2 antibody
    • PTX2 antibody
    • RGS antibody
    • RIEG 1 antibody
    • RIEG antibody
    • Rieg bicoid related homeobox transcription factor 1 antibody
    • RIEG bicoid related homeobox transcription factor antibody
    • RIEG bicoid-related homeobox transcription factor antibody
    • RIEG1 antibody
    • RS antibody
    • Solurshin antibody
    see all

Anti-PITX2 antibody images

  • Anti-PITX2 antibody (ab32832) at 2.5 µg/ml + HepG2 cell lysate

    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size : 35 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling PITX2 with ab32832 at 4-8µg/ml. Cells with positive label (as indicated by the arrows) are epithelial cells of the intestinal villus.

References for Anti-PITX2 antibody (ab32832)

ab32832 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Chicken Tissue sections (Neural tube)
Specification Neural tube
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Dec 21 2011

DISCOUNT CODE: *** Expiration date: January 31, 2012 I am pleased to hear you would like to test ab32832 in chicken tissue. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abrev...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Skeletal muscle)
Loading amount 10 µg
Specification Skeletal muscle
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C

Abcam user community

Verified customer

Submitted May 11 2011