Our Abpromise guarantee covers the use of ab32832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2.5 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use at an assay dependent concentration.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling PITX2 with ab32832 at 4-8µg/ml. Cells with positive label (as indicated by the arrows) are epithelial cells of the intestinal villus.
ab32832 has not yet been referenced specifically in any publications.