Overview

  • Product nameAnti-PITX2 antibody
    See all PITX2 primary antibodies
  • Description
    Rabbit polyclonal to PITX2
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Dog
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human PITX2.

  • Positive control
    • This antibody gave a positive signal in Human Heart Tissue Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab98297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).

Target

  • FunctionMay play an important role in development and maintenance of anterior structures. Isoform PTX2C is involved in left-right asymmetry the developing embryo.
  • Involvement in diseaseDefects in PITX2 are the cause of Axenfeld-Rieger syndrome type 1 (RIEG1) [MIM:180500]; also known as Rieger syndrome type 1. RIEG1 is an autosomal dominant defect characterized by hypodontia (partial anodontia), anal stenosis, hypertelorism, mental deficiency, agenesis of the facial bones, with malformation of the anterior chamber of the eye.
    Defects in PITX2 are the cause of iridogoniodysgenesis type 2 (IRID2) [MIM:137600]; also known as iridogoniodysgenesis syndrome 2 (IGDS2). It is an autosomal dominant inherited disease.
    Defects in PITX2 are a cause of Peters anomaly (PAN) [MIM:604229]. It is a congenital defect of the anterior chamber of the eye.
    Defects in PITX2 are associated with ring dermoid of cornea (RDC) [MIM:180550]. RDC is an autosomal dominantly inherited syndrome characterized by bilateral annular limbal dermoids with corneal and conjunctival extension.
  • Sequence similaritiesBelongs to the paired homeobox family. Bicoid subfamily.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • All1 responsive gene 1 antibody
    • ALL1 responsive protein ARP1 antibody
    • ALL1-responsive protein ARP1 antibody
    • ARP 1 antibody
    • ARP1 antibody
    • Brx 1 antibody
    • Brx1 antibody
    • Homeobox protein PITX2 antibody
    • IDG 2 antibody
    • IDG2 antibody
    • IGDS 2 antibody
    • IGDS antibody
    • IGDS2 antibody
    • IHG 2 antibody
    • IHG2 antibody
    • IRID 2 antibody
    • IRID2 antibody
    • MGC111022 antibody
    • MGC20144 antibody
    • Otlx 2 antibody
    • Otlx2 antibody
    • Paired like homeodomain transcription factor 2 antibody
    • Paired-like homeodomain transcription factor 2 antibody
    • Pituitary homeo box 2 antibody
    • Pituitary homeobox 2 antibody
    • PITX 2 antibody
    • pitx2 antibody
    • PITX2_HUMAN antibody
    • PTX 2 antibody
    • PTX2 antibody
    • RGS antibody
    • RIEG 1 antibody
    • RIEG antibody
    • Rieg bicoid related homeobox transcription factor 1 antibody
    • RIEG bicoid related homeobox transcription factor antibody
    • RIEG bicoid-related homeobox transcription factor antibody
    • RIEG1 antibody
    • RS antibody
    • Solurshin antibody
    see all

Anti-PITX2 antibody images

  • Anti-PITX2 antibody (ab98297) at 1 µg/ml + Human heart tissue lysate - total protein (ab29431) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 35 kDa
    Additional bands at : 55 kDa,60 kDa,85 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • ICC/IF image of ab98297 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab98297 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in methanol fixed (100%, 5min) HepG2, HEK293, and MCF-7 cell lines at 1ug/ml

References for Anti-PITX2 antibody (ab98297)

ab98297 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application ChIP
Sample Mouse Tissue lysate - whole (brain)
Negative control parallel ChIP without antibody
Specification brain
Detection step Semiquantitative PCR
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Positive control none
Username

Dr. Joomyeong Kim

Verified customer

Submitted Jan 21 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"