Anti-PITX3 antibody - Neuronal Marker (ab30734)

Overview

  • Product nameAnti-PITX3 antibody - Neuronal Marker
    See all PITX3 primary antibodies
  • Description
    Rabbit polyclonal to PITX3 - Neuronal Marker
  • Tested applicationsSuitable for: IHC-P, WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse PITX3.

    (Peptide available as ab31696.)

  • Positive control
    • ab30734 gave a positive signal in the following whole cell lysates: Y79; U-87 MG; Hek293
  • General notesWestern blot blocking was performed with 5% milk.

Properties

Applications

Our Abpromise guarantee covers the use of ab30734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 21362567
WB 1/250. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa). Abcam recommends using 5% milk as the blocking agent.
IHC-FoFr Use at an assay dependent concentration.

Target

  • FunctionTranscriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. In addition to its importance during development, it also has roles in the long-term survival and maintenance of the mdDA neurons. Activates NR4A2/NURR1-mediated transcription of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. Acts by decreasing the interaction of NR4A2/NURR1 with the corepressor NCOR2/SMRT which acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state. Essential for the normal lens development and differentiation. Plays a critical role in the maintenance of mitotic activity of lens epithelial cells, fiber cell differentiation and in the control of the temporal and spatial activation of fiber cell-specific crystallins. Positively regulates FOXE3 expression and negatively regulates PROX1 in the anterior lens epithelium, preventing activation of CDKN1B/P27Kip1 and CDKN1C/P57Kip2 and thus maintains lens epithelial cells in cell cycle.
  • Tissue specificityHighly expressed in developing eye lens.
  • Involvement in diseaseDefects in PITX3 are a cause of cataract autosomal dominant (ADC) [MIM:604219]. Cataract is an opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. Cataract is the most common treatable cause of visual disability in childhood.
    Defects in PITX3 are a cause of anterior segment mesenchymal dysgenesis (ASMD) [MIM:107250]; also known as anterior segment ocular dysgenesis (ASOD). ASMD consists of a range of developmental defects in structures at the front of the eye, resulting from abnormal migration or differentiation of the neural crest derived mesenchymal cells that give rise to the cornea, iris, and other components of the anterior chamber during eye development. Mature anterior segment anomalies are associated with an increased risk of glaucoma and corneal opacity. Conditions falling within the phenotypic spectrum include aniridia, posterior embryotoxon, Axenfeld anomaly, Reiger anomaly/syndrome, Peters anomaly, and iridogoniodysgenesis.
    Defects in PITX3 are the cause of cataract posterior polar type 4 (CTPP4) [MIM:610623]. A subcapsular opacity, usually disk-shaped, located at the back of the lens. It can have a marked effect on visual acuity. Some patients affected by cataract posterior polar type 4 can present a severe phenotype including microphthalmia and neurological dysfunction.
  • Sequence similaritiesBelongs to the paired homeobox family. Bicoid subfamily.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Homeobox protein PITX 3 antibody
    • Homeobox protein PITX3 antibody
    • MGC12766 antibody
    • Paired like homeodomain transcription factor 3 antibody
    • Paired-like homeodomain transcription factor 3 antibody
    • Pituitary homeobox 3 antibody
    • PITX 3 antibody
    • Pitx3 antibody
    • PITX3_HUMAN antibody
    • PTX 3 antibody
    • PTX3 antibody
    see all

Anti-PITX3 antibody - Neuronal Marker images

  • All lanes : Anti-PITX3 antibody - Neuronal Marker (ab30734) at 1 µg/ml (5% Milk block)

    Lane 1 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
    Lane 2 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 32 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 160 kDa,26 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • ab30734 staining PITX3 in mouse brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/300 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An abcam antibody ab60314, Chromeo 488 conjugated goat polyclonal to rabbit IgG, diluted 1/1000 was used as secondary.

    See Abreview

References for Anti-PITX3 antibody - Neuronal Marker (ab30734)

This product has been referenced in:
  • Volpicelli F  et al. Direct regulation of Pitx3 expression by Nurr1 in culture and in developing mouse midbrain. PLoS One 7:e30661 (2012). WB ; Mouse . Read more (PubMed: 22363463) »
  • Nguyen HN  et al. LRRK2 mutant iPSC-derived DA neurons demonstrate increased susceptibility to oxidative stress. Cell Stem Cell 8:267-80 (2011). WB, IHC-P ; Human . Read more (PubMed: 21362567) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Brain
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Jan 08 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Brain
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Jan 08 2016



Concerning Anti-PITX3 antibody - Neuronal Marker (ab30734) and Anti-PITX3 antibody [EPR8868] (ab134920) I am happy to be able to offer you a blocking peptide for both of the antibodies: ab31696.

http://www.abcam.com/index.html?datash...

Read More
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Midbrain section)
Specification Midbrain section
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Apr 07 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

Submitted Jan 28 2009

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (brain sections)
Specification brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

Submitted Oct 13 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"