Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse PITX3.
(Peptide available as ab31696.)
Our Abpromise guarantee covers the use of ab30734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. PubMed: 21362567|
|WB||1/250. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa). Abcam recommends using 5% milk as the blocking agent.|
|IHC-FoFr||Use at an assay dependent concentration.|
ab30734 staining PITX3 in mouse brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/300 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An abcam antibody ab60314, Chromeo 488 conjugated goat polyclonal to rabbit IgG, diluted 1/1000 was used as secondary.